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Method for cultivating cordyceps militaris rich in SOD (superoxide dismutase)

A cultivation method, technology of Cordyceps militaris, applied in botany equipment and method, fertilizer mixture, gardening, etc.

Active Publication Date: 2011-09-14
湖北圣峰药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the culture medium of artificially cultivating Cordyceps militaris, the content of Cu and Zn elements is very small, and the existing techniques for cultivating Cordyceps militaris do not add copper and zinc salts necessary for transforming and synthesizing SOD in the culture medium. The SOD specific activity does not exceed 20 u / mg

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of Copper Acid, Zinc Liquid:

[0020] Weigh 1 gram of copper glycinate and 1 gram of zinc glycinate, dissolve them in 1000 milliliters of water, and set aside;

[0021] (2) Preparation of nutrient solution:

[0022] Weigh 10 grams of glucose, 10 grams of peptone, 1 gram of potassium dihydrogen phosphate, and 4 milliliters of copper-zinc glycine solution, add them to an appropriate amount of hot water, stir and dissolve, and add 1000 milliliters of water to make up;

[0023] (3) Preparation of nutrient base

[0024] Take 50 grams of rice and 80 ml of nutrient solution into a 600 ml wide-mouth glass bottle, seal the bottle with polypropylene plastic film and put it in an autoclave, sterilize at 126°C for 40 minutes, wait until the pressure drops to Take it out of the pot under normal pressure, and let it cool down to room temperature naturally;

[0025] (4) Inoculation

[0026] Move the culture bottle to the ultra-clean bench, open the plastic film of ...

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Abstract

The invention relates to a method for cultivating cordyceps militaris rich in SOD (superoxide dismutase). By adding copper-glycine and zinc glycinate into a culture medium nutrient solution, a material base which is necessary for transformation and synthesis of the SOD in the growing process is provided for the cordyceps militaris, so that the SOD specific activity of the cultivated cordyceps militaris entity can be compared favourably with wild cordyceps militaris and reaches over 50u / mg.

Description

technical field [0001] The invention relates to a cultivation method of Cordyceps militaris rich in SOD. Background technique [0002] Superoxide dismutase (SOD) is a metalloenzyme that exists in organisms to specifically scavenge superoxide anion free radicals. SOD plays a very important role in defending against oxygen poisoning, resisting radiation damage and preventing aging. Free radicals in the human body can kill germs and microorganisms that invade the body and protect the body. But when there are too many free radicals, they attack normal cells in the body just like bacteria, causing cells to be damaged and destroyed, and damage the genetic factors DNA in cells, thus causing premature aging and a series of diseases. The SOD in the body can remove excessive free radicals, but the SOD produced in the body gradually decreases after middle age, the SOD activity decreases, and the body ages. Exogenous supplementation of SOD has a significant effect on preventing diseas...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 徐景辉
Owner 湖北圣峰药业有限公司
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