Research on technology for artificially modifying extended-spectrum bacteriophage

Abstract

The invention discloses a method for artificially modifying a bacteriophage to extend a host spectrum of the bacteriophage, belongs to the field of food safety detection, and relates to exploratory application of a biotechnology to the food safety detection. The method comprises the following steps of: screening wild virulent bacteriophages, screening a virulent bacteriophage (K1) with a wider lysis spectrum, artificially modifying a genome of the bacteriophage, returning the knocked-out genome to the original bacteriophage by taking Escherichia coli as an intermediate vector, namely returning the modified genome to the original bacteriophage by the Escherichia coli top10, and detecting a phagocytosis spectrum of the modified bacteriophage. By the method, one modified bacteriophage with a wide lysis spectrum is screened and obtained; and experimental results show that the bacteriophage can synchronously detect five food-borne pathogenic bacteria, the detection time is 3 hours, the titer is 1*10<12>CFU / ml, and the detection sensitivity is 95.3 percent.

Description

technical field [0001] The invention belongs to the field of food safety detection and is an exploratory application of biotechnology in food safety detection. Background technique [0002] In recent years, food safety issues have become increasingly prominent, among which foodborne diseases caused by foodborne pathogenic bacteria have become one of the most serious public health problems, which not only endanger the vital interests of the broad masses of the people, but also affect the economic Sustainable development and social harmony and stability. The increasingly serious hazard situation of food-borne pathogens reminds us that the current detection methods of food-borne pathogens cannot fully meet the needs of the current food safety inspection work, and it is necessary to further establish and improve effective food safety inspection technology and monitoring system , where the most important task is to establish a high-throughput, rapid and sensitive detection metho...

AI Technical Summary

Problems solved by technology

The increasingly serious hazard situation of food-borne pathogens reminds us that the current detection methods of food-borne pathogens cannot fully meet the needs of the current food safety inspection work, and it is necessary to further establish and improve effective food safety inspection technology and monitoring system , the most important task is to establish a high-throughput, rapid and sensitive detection method