Respiratory virus detection kit and detection method
A virus detection and respiratory technology, applied in the field of molecular biology, can solve the problems of long detection time, inability to cope with respiratory virus detection, and low sensitivity, and achieve the effects of sensitivity, specificity, strong practicability, and high detection throughput
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Embodiment 1
[0078] Design and prepare primers and probes
[0079] By analyzing the gene sequences of 8 common respiratory viruses, a relatively conservative sequence is selected as the target amplification fragment, and PCR amplification primers for amplifying the fragment and specifically binding to the fragment are designed according to the target amplification fragment the probe.
[0080] The 8 target amplification fragments and their corresponding PCR amplification primers and probes are as follows:
[0081] The first target amplified fragment is the nucleic acid fragment shown in SEQ ID NO.1 of the gene of SARS virus, the corresponding upstream primer sequence is shown in SEQ ID NO.9, and the downstream primer sequence is shown in SEQ ID NO.17 , the probe sequence is shown in SEQ ID NO.25.
[0082] The second target amplified fragment is the nucleic acid fragment shown in SEQ ID NO.2 of the gene of influenza A virus, the corresponding upstream primer sequence is shown in SEQ ID NO....
Embodiment 2
[0093] Respiratory virus detection kit and detection method
[0094] The detection kit includes the above-mentioned 8 sets of primers and 8 probes.
[0095] In a preferred embodiment, the detection kit also includes a positive quality control probe and a positive probe complementary to the positive quality control probe; the sequence of the positive quality control probe is shown in SEQ ID NO.33, and the positive probe's The sequence is shown in SEQ ID NO.34.
[0096] In this example, the 5' end of the positive probe was labeled with biotin.
[0097] Such as figure 1 The detection method of shown respiratory virus comprises the steps:
[0098] S10, extracting the nucleic acid in the sample to be tested
[0099] Nucleic acid was extracted using the column virus RNAOUT extraction kit (product number 71001-50) of Beijing Tianenze Gene Technology Co., Ltd.
[0100] The usage method is as follows:
[0101] 1. For liquid virus samples: Add 0.1-0.2 mL of liquid virus samples in...
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