Protein chromatography system

Abstract

The invention relates to a protein chromatography system, which comprises a plurality of chromatography columns and at least one main trunk pipeline, wherein the front part and the rear part of each chromatography column are respectively connected with the main trunk pipelines through electric switching valves; and the main trunk pipelines are also communicated with cleaning agents through cleaning pumps. At least two main trunk pipelines are respectively connected to a total trunk pipeline through the electric switching valves to form a set of combined multiple systems. The cleaning agents can be periodically pumped into the main trunk pipelines through the cleaning pumps of the main trunk pipelines, thereby keeping the main trunk pipelines clean so as to ensure that the protein is prevented from being polluted by pyrogen and other substances in the separation process of the protein, or mutual cross contamination among multiple uses is avoided. According to the system disclosed by the invention, a plurality of columns can simultaneously work, and therefore the process time is shortened, and the handling capacity and the speed of the equipment are increased; the separation and the purification of the protein and various biological macromolecules can be quickly carried out; and protein drugs, enzymes, antibodies, vaccines and other products can be acquired from animals and plants, microorganisms and genetic engineering fermentation products.

Description

technical field [0001] The invention relates to separation and purification equipment for bioengineering products, in particular to a protein chromatography system combined with high-throughput multi-layer chromatography columns. Background technique [0002] Chromatography is a unit operation often used in modern chemical engineering and biochemical separation processes. For protein mixtures with complex components (such as plasma products), it is difficult to separate the active components, the process is complex, time-consuming, poor resolution, and low efficiency. Multiple columns and a variety of complementary chromatographic modes, including ion exchange, hydrophobic, affinity, gel filtration, and reversed-phase, are often used in the separation process. Using a traditional protein chromatography system, in routine operations, the components separated by each chromatography column need to be collected and then injected into the next chromatography column. During this ...

AI Technical Summary

Problems solved by technology

Using a traditional protein chromatography system, in routine operations, the components separated by each chromatography column need to be collected and then injected into the next chromatography column. During this process, manual column replacement is required, resulting in a high separation speed. Slow, cumbersome and complicated operation, and the sample is exposed to the air, which is easy to cause pollution and loss of activity

[0003] At present, the traditional protein chromatography systems of domestic and foreign companies have problems such as cumbersome column disassembly and difficult combination of multi-column chromatography in the replacement of different separation modes, and have defects in flow rate range, scalability, and column compatibility.

[0004] In recent years, some multi-column combined chromatography systems have been proposed at home and abroad. These systems can be summarized into two categories: 1) Parallel multi-column chromatography systems, where multiple chromatography columns are connected in parallel on a single system, and all chromatography columns work simultaneously. , but the same operation is carried out, as disclosed in Chinese Patent No. 200720073590.6, this type of system can only improve the throughput of chromatography, but cannot achieve the separation of multiple modes; 2) series multi-column chromatography system, layer The separation columns are directly switched through the electric switching valve, that is, the components are directly injected into another chromatography column through the electric switching valve from one chromatography column. As disclosed in Chinese patent application number 200910131502.7, each chromatography column It can only be operated in sequence, and only one chromatography column is working at the same time. Usually, only two to three chromatography columns can be connected to this type of system, and the chromatography modes that can be used are limited.

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