Method for extracting tea-seed oil and tea saponin by using biological method

A technology for tea seed oil and tea saponin, which is applied in the field of extracting tea seed oil and tea saponin by biological methods, can solve the problems of affecting the health of consumers, low oil yield in the pressing process, deepening oil color, etc. Inhibits the growth of bacteria and mold, mild working conditions, and prevents oxidation

Inactive Publication Date: 2012-10-10
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the extraction of tea seed oil and tea saponin in the industry is carried out separately. First, the tea seed oil is extracted by pressing or organic solvents, and then the tea saponin is extracted from the tea seed meal after oil extraction. The process is very cumbersome
Moreover, the oil yield rate of the pressing process is not high, and the Maillard reaction will occur during the oil extraction process to deepen the color of the oil; when using organic solvent extraction, a desolventization process is required after solvent leaching, with many equipment, large investment, and heavy pollution. There will always be a small amount of solvent remaining in the camellia oil and its residue, which will affect the health of the eater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The tea seed kernels of 50kg were steamed, fried and pulverized to 10 μm; the tap water of 200kg was added, and the temperature was raised to 60° C., and adjusting pH was 6.0, and then added 50g α-amylase (medium temperature amylase produced by Novozymes, and the enzymatic activity was 9000u / g), enzymolysis for 2h; after obtaining the enzymolysis solution, lower the temperature to 50℃, adjust the pH to 4.0, add 50g saccharification enzyme (manufactured by Novozymes, the enzyme activity is 100000u / ml), enzymolysis for 2h; enzymolysis After finishing, adjust the pH of the solution to 6.5, centrifuge to obtain oil phase and water phase; separate the oil phase after centrifugation through an ultrafiltration membrane with a pressure of 0.2MPa and a temperature of 45°C to obtain crude oil, and then add lye Deacidification to obtain 19.5kg of tea seed oil of higher purity; at the same time, the pH of the centrifuged aqueous phase was adjusted to 2.5, and centrifugation was perf...

Embodiment 2

[0029] 50kg of tea seed kernels were steamed, fried and pulverized to 10 μm; 200kg of tap water was added, the temperature was raised to 70° C., the pH was adjusted to 6.5, and 75g of α-amylase (medium-temperature amylase produced by Novozymes, with an enzymatic activity of 9000u) was added. / g), enzymatic hydrolysis for 3 hours; after obtaining the enzymatic hydrolysis solution, lower the temperature to 55 °C, adjust the pH to 4.5, add 75 g of saccharification enzyme (manufactured by Novozymes, the enzyme activity is 100000u / ml), enzymatic hydrolysis for 3 hours; the enzymatic hydrolysis is over Then, adjust the pH of the solution to 6.5, centrifuge to obtain oil phase and water phase; separate the oil phase after centrifugation through an ultrafiltration membrane with a pressure of 0.2 MPa and a temperature of 50 °C to obtain crude oil, and then add alkali solution to remove the oil. acid to obtain 19.82kg of high-purity tea seed oil; also adjust the pH of the centrifuged aqu...

Embodiment 3

[0031] The tea seed kernels of 50kg were steamed, fried and pulverized to 10 μm; the tap water of 200kg was added, and the temperature was raised to 80° C., adjusting pH was 7, and then adding 100g α-amylase (medium temperature amylase produced by Novozymes, the enzymatic activity was 9000u / g), enzymatic hydrolysis for 3 hours; after obtaining the enzymatic hydrolysis solution, lower the temperature to 60 °C, adjust the pH to 5.0, add 100 g of saccharification enzyme (manufactured by Novozymes, the enzyme activity is 100000u / ml), enzymatic hydrolysis for 3 hours; enzymatic hydrolysis After finishing, adjust the pH of the solution to 6.5, centrifuge to obtain oil phase and water phase; separate the oil phase after centrifugation through an ultrafiltration membrane with a pressure of 0.3 MPa and a temperature of 50 ° C to obtain crude oil, and then add lye Deacidification to obtain 20.03kg of tea seed oil of higher purity; at the same time, the pH of the centrifuged aqueous phase...

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Abstract

The invention relates to a method for extracting tea saponin and tea-seed oil from tea seed kernels by using a biological method. The amount of each added material is based on the weight of the tea seed kernels, and the method comprises the following steps of: cooking the tea seed kernels, crushing, liquefying and saccharifying, adjusting the pH, and performing centrifugation; for the obtained supernatant oil comprising the main components of fat and oligosaccharide, performing membrane separation to obtain crude oil, and performing alkaline refining de-acidification to obtain refined tea oil; for the subnatant water phase comprising the main components of saponin and protein, adjusting the pH, and then performing centrifugation to obtain crude tea saponin; and performing decontaminating concentration by using an ultrafiltration membrane to obtain refined tea saponin. According to the method, the tea seed kernels are reasonably used to extract the tea-seed oil and the tea saponin at the same time, the process is simple, the operation is safe, high-purity tea oil and saponin are obtained, and simultaneously secondary pollution and a great deal of wastewater cannot be generated.

Description

technical field [0001] The invention relates to a production method for comprehensively processing tea seed kernels by using enzymatic hydrolysis technology, and mainly relates to a method for simultaneously extracting tea seed oil and tea saponin from the tea seed kernels. Background technique [0002] Camellia Oleifera Abel. (Camellia Oleifera Abel.) is an evergreen, long-lived tree species with high oil content in fruit. It has a long history of cultivation and is one of the two major woody edible oils in the world. Camellia oleifera, also known as camellia tree, has a fruit growth period of more than 400 days, which is the longest bred by nature among all oil crops. It has extremely high nutritional value and has been regarded as a mountain tribute since ancient times. my country is both the origin and main producing area of ​​Camellia oleifera. At present, the area of ​​Camellia oleifera forests in the country is about 4 million hectares, accounting for more than 80% of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00C11B1/04C11B3/00C11B3/06A23D9/02C07J63/00C07H15/256C07H1/08
Inventor 万端极徐国念吴正奇毛波李猷
Owner 武汉普赛特膜技术循环利用有限公司
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