The invention relates to a comprehensive utilization method of a pumpkin. In the method, added amount of substances is based on weight of the pumpkin; and the pumpkin is taken as a raw material for step-by-step extraction to respectively obtain pumpkin polysaccharide, pectin and pumpkin powder by utilizing a biotechnology and a membrane separation technology. The method comprises the following steps: firstly washing and cutting the pumpkin into blocks, removing seeds, pulping, carrying out water extraction at moderate temperature and centrifugal separation, carrying out acid precipitation on the obtained water extract, concentrating the obtained supernate through a nanofiltration membrane, and spray-drying to obtain the polysaccharide; extracting residues after water extraction by utilizing acid water, carrying out centrifugal separation, removing impurities from the obtained supernate through a microfiltration membrane, concentrating the supernate through a nanofiltration membrane, carrying out acid precipitation on the obtained concentrated solution, carrying out centrifugal separation, and freeze-drying the obtained precipitate to obtain the pectin; spray-drying the supernate to obtain the polysaccharide; and mixing the remaining residues and filtered liquor, grinding by a colloid mill and spray-drying to obtain the pumpkin powder. The comprehensive utilization method has the advantages that effective substances of the pumpkin are fully utilized, and process is simple and feasible, thus being applicable to industrialized production.