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Method for extracting tea-seed oil and tea saponin by using biological method

A technology for tea saponin and tea seed oil, which is applied in the field of extracting tea seed oil and tea saponin by biological method, can solve the problems of affecting the health of consumers, low oil yield in the pressing process, deepening oil color, etc. Simplified degumming and deacidification, mild working conditions, and the effect of reducing impurities

Inactive Publication Date: 2011-09-21
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the extraction of tea seed oil and tea saponin in the industry is carried out separately. First, the tea seed oil is extracted by pressing or organic solvents, and then the tea saponin is extracted from the tea seed meal after oil extraction. The process is very cumbersome
Moreover, the oil yield rate of the pressing process is not high, and the Maillard reaction will occur during the oil extraction process to deepen the color of the oil; when using organic solvent extraction, a desolventization process is required after solvent leaching, with many equipment, large investment, and heavy pollution. There will always be a small amount of solvent remaining in the camellia oil and its residue, which will affect the health of the eater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 50kg of tea seed kernels were steamed, fried, and crushed to 10 μm; 200kg of tap water was added, the temperature was raised to 60°C, and the pH was adjusted to 6.0, and then 50g of α-amylase (medium-temperature amylase produced by Novozymes, with an enzyme activity of 9000u / g), enzymolysis for 2 hours; after obtaining the enzymolysis solution, lower the temperature to 50°C, adjust the pH to 4.0, add 50g of glucoamylase (manufactured by Novozymes, the enzyme activity is 100000u / ml), and enzymolysis for 2 hours; After the end, adjust the pH of the solution to 6.5, and centrifuge to obtain the oil phase and water phase; the centrifuged oil phase is separated through an ultrafiltration membrane with a pressure of 0.2 MPa and a temperature of 45°C to obtain crude oil, and then add lye Deacidification to obtain 19.5kg of tea seed oil with higher purity; at the same time, adjust the pH of the centrifuged water phase to 2.5, centrifuge to obtain crude saponin, and then pass the...

Embodiment 2

[0029] 50kg of tea seed kernels were steamed, fried, and crushed to 10 μm; 200kg of tap water was added, the temperature was raised to 70°C, the pH was adjusted to 6.5, and 75g of α-amylase (medium temperature amylase produced by Novozymes, with an enzyme activity of 9000u / g), enzymatic hydrolysis for 3 hours; after obtaining the enzymatic hydrolysis solution, lower the temperature to 55°C, adjust the pH to 4.5, add 75g of glucoamylase (manufactured by Novozymes, the enzyme activity is 100000u / ml), and enzymatic hydrolysis for 3 hours; the end of enzymatic hydrolysis Finally, adjust the pH of the solution to 6.5, and centrifuge to obtain an oil phase and a water phase; the centrifuged oil phase is separated through an ultrafiltration membrane with a pressure of 0.2 MPa and a temperature of 50°C to obtain crude oil, and then add lye to remove acid to obtain 19.82kg of tea seed oil with higher purity; meanwhile, adjust the pH of the centrifuged water phase to 2.5, centrifuge to ...

Embodiment 3

[0031] 50kg of tea seed kernels were steamed, fried, and crushed to 10 μm; 200kg of tap water was added, the temperature was raised to 80°C, and the pH was adjusted to 7, and then 100g of α-amylase (medium-temperature amylase produced by Novozymes, with an enzyme activity of 9000u / g), enzymolysis for 3 hours; after obtaining the enzymolysis solution, lower the temperature to 60°C, adjust the pH to 5.0, add 100g of glucoamylase (manufactured by Novozymes, the enzyme activity is 100000u / ml), and enzymolysis for 3 hours; enzymolysis After the end, adjust the pH of the solution to 6.5, and centrifuge to obtain the oil phase and water phase; the centrifuged oil phase is separated through an ultrafiltration membrane with a pressure of 0.3MPa and a temperature of 50°C to obtain crude oil, and then add lye Deacidification to obtain 20.03kg of high-purity tea seed oil; at the same time, adjust the pH of the centrifuged water phase to 2.5, centrifuge to obtain crude saponin, and then pas...

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Abstract

The invention relates to a method for extracting tea saponin and tea-seed oil from tea seed kernels by using a biological method. The amount of each added material is based on the weight of the tea seed kernels, and the method comprises the following steps of: cooking the tea seed kernels, crushing, liquefying and saccharifying, adjusting the pH, and performing centrifugation; for the obtained supernatant oil comprising the main components of fat and oligosaccharide, performing membrane separation to obtain crude oil, and performing alkaline refining de-acidification to obtain refined tea oil; for the subnatant water phase comprising the main components of saponin and protein, adjusting the pH, and then performing centrifugation to obtain crude tea saponin; and performing decontaminating concentration by using an ultrafiltration membrane to obtain refined tea saponin. According to the method, the tea seed kernels are reasonably used to extract the tea-seed oil and the tea saponin at the same time, the process is simple, the operation is safe, high-purity tea oil and saponin are obtained, and simultaneously secondary pollution and a great deal of wastewater cannot be generated.

Description

technical field [0001] The invention relates to a production method for comprehensively processing tea seed kernels by adopting enzymatic hydrolysis technology, and mainly relates to a method for synchronously extracting tea seed oil and tea saponin from tea seed kernels. Background technique [0002] Camellia oleifera (Camellia Oleifera Abel.) is an evergreen, long-lived tree species with high fruit oil content. It has a long history of cultivation and is one of the two largest woody edible oil plants in the world. Camellia oleifera, also known as camellia, has a fruit growth period of more than 400 days. It is the longest of all oil crops to be conceived by nature. It has high nutritional value and has been regarded as a mountain tribute since ancient times. my country is not only the origin of camellia oleifera, but also the main place of origin. At present, the area of ​​camellia oleifera forests in the country is about 4 million hectares, accounting for more than 80% of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C11B3/00C11B3/06A23D9/02C07J63/00C07H15/256C07H1/08
Inventor 万端极徐国念吴正奇毛波李猷
Owner 武汉普赛特膜技术循环利用有限公司
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