Method for producing purely natural monosodium glutamate by using low-value marine fishes
A marine fish and low-value technology is applied in the field of using low-value marine fish to produce pure natural monosodium glutamate. Effect
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Embodiment 1
[0030] Soak 20kg of low-value marine fish in 20kg of water, and add 4g of antioxidant BHA at the same time; raise the temperature to 70°C, adjust the pH value to 8, and stir for 1 hour at a temperature of 60°C; add 60g of alkaline protease, alkaline The protease is produced by Novozymes, Qingdao, Shandong, with an enzyme activity of 50,000 U / g; at a pH value of 7 and a temperature of 50°C, the enzymolysis is performed for 2 hours; after the enzymolysis is complete, the enzymolysis solution is filtered to remove fishbones and bones. Then centrifuge to separate the fish oil and fish dregs; then add 60g of flavor enzyme to the filtrate at a pH of 7 and an enzymolysis temperature of 50°C. g, enzymatic hydrolysis for 2 hours; after enzymatic hydrolysis, heat to 100°C and keep for 5 minutes to inactivate the enzyme; adjust the pH of the enzymolysis solution to 5.5 with citric acid, add 200g of activated carbon, stir at 60°C for 2h, and centrifuge. Filtration; then the filtrate is pa...
Embodiment 2
[0032] Soak 20kg of low-value marine fish in 40kg of water, and add 2g of antioxidant BHT at the same time; raise the temperature to 75°C, adjust the pH value to 8.5, and stir for 2 hours at a temperature of 70°C; add 80g of alkaline protease, alkaline The protease is produced by Novozymes in Qingdao, Shandong Province, with an enzyme activity of 200,000 U / g. It is hydrolyzed for 3 hours at a pH value of 8 and a temperature of 60°C; Then centrifuge to separate the fish oil and fish dregs; then add 100g of flavor enzyme to the filtrate when the pH value is 7 and the enzymatic hydrolysis temperature is 55°C. g, enzymatic hydrolysis for 3 hours; after enzymatic hydrolysis, heat to 100°C and keep for 5 minutes to inactivate the enzyme; adjust the pH of the enzymolysis solution to 6 with citric acid, add 300g of activated carbon, stir at 70°C for 3h, centrifuge, Filtrate; then pass the filtrate through an ultrafiltration membrane with a molecular weight cut-off of 2000Dal to remove...
Embodiment 3
[0034] Soak 20kg of low-value marine fish in 60kg, and add 6g of antioxidant TBHQ at the same time; quickly raise the temperature to 75°C, adjust the pH value to 8.5, and stir for 2h at a temperature of 65°C; after 2h, add 100g of alkaline protease , Alkaline protease is produced by Novozymes, Qingdao, Shandong, with an enzyme activity of 200,000 U / g. At a pH value of 9 and a temperature of 60°C, enzymolyze for 3 hours; after the enzymolysis is complete, filter the enzymolysis solution to remove fish bones , bone, and then centrifuged to separate fish oil and fish dregs; when the pH value of the filtrate was 8 and the enzymolysis temperature was 55°C, 80 g of flavor enzyme was added. The flavor enzyme was produced by Novozymes, Qingdao, Shandong, and the enzyme activity was 10 10,000 U / g, enzymatic hydrolysis for 3 hours; after enzymatic hydrolysis, heat to 100°C and keep for 5 minutes to inactivate the enzyme; adjust the pH of the enzymatic hydrolysis solution to 6 with citric...
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