Method for producing purely natural monosodium glutamate by using low-value marine fishes

A marine fish and low-value technology is applied in the field of using low-value marine fish to produce pure natural monosodium glutamate. Effect

Active Publication Date: 2011-03-16
WUHAN AIMIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The umami taste of monosodium glutamate is prominent, but the umami taste is single and the aftertaste is short, especially sodium glutamate produces sodium pyroglutamate under high temperature conditions, and adverse reactions such as dizziness, dizziness, and vomiting will occur after eating
[0004] At present, monosodium glutamate is made from grain raw materials by microbial fermentation. Its main disadvantages are: high cost of raw materials, difficulty in controlling the fermentation process, large amount of waste water, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Soak 20kg of low-value marine fish in 20kg of water, and add 4g of antioxidant BHA at the same time; raise the temperature to 70°C, adjust the pH value to 8, and stir for 1 hour at a temperature of 60°C; add 60g of alkaline protease, alkaline The protease is produced by Novozymes, Qingdao, Shandong, with an enzyme activity of 50,000 U / g; at a pH value of 7 and a temperature of 50°C, the enzymolysis is performed for 2 hours; after the enzymolysis is complete, the enzymolysis solution is filtered to remove fishbones and bones. Then centrifuge to separate the fish oil and fish dregs; then add 60g of flavor enzyme to the filtrate at a pH of 7 and an enzymolysis temperature of 50°C. g, enzymatic hydrolysis for 2 hours; after enzymatic hydrolysis, heat to 100°C and keep for 5 minutes to inactivate the enzyme; adjust the pH of the enzymolysis solution to 5.5 with citric acid, add 200g of activated carbon, stir at 60°C for 2h, and centrifuge. Filtration; then the filtrate is pa...

Embodiment 2

[0032] Soak 20kg of low-value marine fish in 40kg of water, and add 2g of antioxidant BHT at the same time; raise the temperature to 75°C, adjust the pH value to 8.5, and stir for 2 hours at a temperature of 70°C; add 80g of alkaline protease, alkaline The protease is produced by Novozymes in Qingdao, Shandong Province, with an enzyme activity of 200,000 U / g. It is hydrolyzed for 3 hours at a pH value of 8 and a temperature of 60°C; Then centrifuge to separate the fish oil and fish dregs; then add 100g of flavor enzyme to the filtrate when the pH value is 7 and the enzymatic hydrolysis temperature is 55°C. g, enzymatic hydrolysis for 3 hours; after enzymatic hydrolysis, heat to 100°C and keep for 5 minutes to inactivate the enzyme; adjust the pH of the enzymolysis solution to 6 with citric acid, add 300g of activated carbon, stir at 70°C for 3h, centrifuge, Filtrate; then pass the filtrate through an ultrafiltration membrane with a molecular weight cut-off of 2000Dal to remove...

Embodiment 3

[0034] Soak 20kg of low-value marine fish in 60kg, and add 6g of antioxidant TBHQ at the same time; quickly raise the temperature to 75°C, adjust the pH value to 8.5, and stir for 2h at a temperature of 65°C; after 2h, add 100g of alkaline protease , Alkaline protease is produced by Novozymes, Qingdao, Shandong, with an enzyme activity of 200,000 U / g. At a pH value of 9 and a temperature of 60°C, enzymolyze for 3 hours; after the enzymolysis is complete, filter the enzymolysis solution to remove fish bones , bone, and then centrifuged to separate fish oil and fish dregs; when the pH value of the filtrate was 8 and the enzymolysis temperature was 55°C, 80 g of flavor enzyme was added. The flavor enzyme was produced by Novozymes, Qingdao, Shandong, and the enzyme activity was 10 10,000 U / g, enzymatic hydrolysis for 3 hours; after enzymatic hydrolysis, heat to 100°C and keep for 5 minutes to inactivate the enzyme; adjust the pH of the enzymatic hydrolysis solution to 6 with citric...

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PUM

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Abstract

The invention relates to a method for producing purely natural monosodium glutamate by using low-value marine fishes, which comprises the following steps of: adding water in an amount which is 1 to 3 times the weight of low-value marine fishes, and 0.01 to 0.03 weight percent of antioxidant into the low-value marine fishes; raising the temperature to 60 to 80 DEG C, stirring at the pH of 7 to 9 for 1 to 3 hours, adding 0.3 to 0.5 weight percent of alkaline protease and performing enzymolysis at the temperature of between 45 and 60 DEG C and the pH of 7 to 9 for 2 to 4 hours; filtering to remove fish bones and centrifuging to remove fish oil and fish scrap; adding 0.3 to 0.5 weight percent of flavorzyme into filtrate and performing enzymolysis at the temperature of between 45 and 60 DEG C and the pH of 6 to 8 for 2 to 4 hours; heating to 100 DEG C after the enzymolysis is finished, and inactivating for 5 minutes; regulating the pH of enzymolysis liquid to 5 to 6.5 by using citric acid, adding 1 to 3 weight percent of activated carbon, stirring at the temperature of between 60 and 75 DEG C for 2 to 3 hours, centrifuging and filtering; removing impurities from filtrate by using an ultrafiltration membrane of about 2,000Dal and concentrating the filtrate by using a nanofiltration membrane of about 400Dal in turn; and adding 0.1 to 0.3 weight percent of flavor substances and 0.2 to 0.5 weight percent of truffle into concentrated solution, dissolving, uniformly mixing and performing spray drying. The low-value marine fishes are reasonably utilized, the process is simple, secondary pollution is avoided and a large amount of waste water cannot be generated.

Description

technical field [0001] The invention relates to a production method of pure natural monosodium glutamate, in particular to a method for preparing a natural monosodium glutamate with no fishy smell and strong seafood flavor by using low-value marine fish as raw materials. Background technique [0002] my country is a large ocean country, and its aquatic product production accounts for about one-third of the world's total output, ranking first in the world. The fishery area is 2.8 million square meters, accounting for 59% of China's total sea area. In 2008, my country's seafood output reached 29.8 million tons, of which fish output was 16.3 million tons, crustacean output was 4.98 million tons, and shellfish output was 12.65 million tons. The output of cephalopods is 1.1 million tons, that of algae is 2.42 million tons, and that of other species is 740,000 tons. In recent years, due to profound changes in my country's fishery structure, a large number of low-value marine fish ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/221A23L1/226A23L27/10A23L27/20
Inventor 万端极徐国念吴正奇毛波万莹王聪王旭刘锐谢鑫
Owner WUHAN AIMIN PHARMA
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