Method for pretreating water hyacinth
A water hyacinth and pretreatment technology, applied in fermentation and other directions, can solve the problems of failing to meet requirements, waste water hyacinth biomass turning waste into treasure, etc., and achieve the effect of high enzymatic hydrolysis efficiency.
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Embodiment 1
[0041] Example 1: Preparation of glucose standard curve
[0042] Glucose is the main product of cellulose hydrolysis. Therefore, by measuring the content of glucose in the enzymatic hydrolysis of water hyacinth samples after different pretreatments, the pros and cons of different pretreatment conditions can be compared. For the determination of the glucose yield in the sample, it is first necessary to prepare a glucose standard curve. The basic process is as follows:
[0043] (1) 1mg / mL glucose standard solution
[0044] Accurately weigh 100 mg of analytically pure glucose baked to constant weight at 80 °C, put it in a small beaker, add a small amount of distilled water to dissolve, transfer it to a 100 mL volumetric flask, dilute to 100 mL with distilled water, mix well, and store in a 4 °C refrigerator for later use .
[0045] (2) 3,5-dinitrosalicylic acid (DNS) reagent
[0046] Add 6.3g DNS and 262mL 2M NaOH solution to 500mL hot aqueous solution containing 185g pot...
Embodiment 2
[0051] Example 2: Determination of cellulose, hemicellulose and lignin content in water hyacinth before pretreatment
[0052] The freshly salvaged water hyacinth material was washed, air-dried, dried at 80°C, and the leaves and roots were pulverized respectively. The 40-mesh sieve components of the leaves were used for the determination of cellulose, hemicellulose and lignin content. Accurately weigh 1.0000g of a sample that has passed through a 40-mesh sieve, and determine the content of each component by Fan's cellulose content method. Before pretreatment, the contents of cellulose, hemicellulose and lignin in the dry matter of water hyacinth were 25.8%, 32.4% and 14.2%, respectively.
Embodiment 3
[0053] Example 3: Pretreatment of water hyacinth
[0054] The water hyacinth components that have passed through a 40-mesh sieve were added with 200 mL of 0.5%, 1%, and 2% H at a solid-liquid ratio of 1:10 (v / v). 2 SO 4 (w / v) and 0.5%, 1%, 2% NaOH (w / v) solution in the Erlenmeyer flask, 100 ℃ for 1h. After cooling, 6 layers of gauze were filtered, the filter residue was washed with water until neutral, and dried at 60°C. The water hyacinth sample was added to a triangular flask containing 200 mL of water at a solid-liquid ratio of 1:10 (v / v), kept at 100 °C for 1 h, and collected as a control. The dried samples were used for fermentation-related quality evaluation.
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