Preparation method of microbial fermentation feed
A microbial fermentation and feed technology, which is applied in the field of livestock and poultry breeding, can solve problems such as uncontrollable temperature, short shelf life, and death of livestock and poultry, and achieve the effects of improving digestion and absorption utilization, improving digestion and absorption functions, and ensuring fermentation quality
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Embodiment 1
[0033] Prepare microbial fermented feed according to the following steps:
[0034] (1) Preparation of fermented product: prepare fermented product according to the following fermented product formula,
[0035] High-quality rice bran 50%, rice 45%, glucose 5%;
[0036] (2) Strain preparation: prepare Lactobacillus strains and brewer's yeast strains in a ratio of 10:1;
[0037] (3) Lactobacillus strain rejuvenation: rejuvenate the lactic acid bacteria strain prepared in step (2), the composition of the rejuvenation medium is as follows: casein peptone 10g / L, broth extract 10g / L, yeast extract Compound 5g / L, glucose 20g / L, Tween 1g / L, K 2 HPO 4 2g / L, sodium acetate 5g / L, diammonium citrate 2g / L, MgSO 4 ·7H 2 O 02g / L, MnSO 4 ·H 2 O 0.05g / L, the pH value of the culture medium is 6.2, the culture temperature of the culture medium is 37 ℃, and the culture time is 1 day;
[0038] (4) Preparation of strain mixture: add 200ml of rejuvenated Lactobacillus strains in step (3) and...
Embodiment 2
[0043] Prepare microbial fermented feed according to the following steps:
[0044] (1) Preparation of fermented product: prepare fermented product according to the following fermented product formula,
[0045] High-quality rice bran 80%, rice 10%, glucose 10%;
[0046] (2) Strain preparation: prepare Lactobacillus strains and brewer's yeast strains in a ratio of 10:4;
[0047] (3) Lactobacillus strain rejuvenation: rejuvenate the lactic acid bacteria strain prepared in step (2), the composition of the rejuvenation medium is as follows: casein peptone 10g / L, broth extract 10g / L, yeast extract Compound 5g / L, glucose 20g / L, Tween 1g / L, K 2 HPO 4 2g / L, sodium acetate 5g / L, diammonium citrate 2g / L, MgSO 4 ·7H 2 O 0.2g / L, MnSO 4 ·H 2 O 0.05g / L, the pH value of medium is 6.5, the culture temperature of culture medium is 42 ℃, and culture time is 2 days;
[0048] (4) Preparation of strain mixture: add 200ml of rejuvenated Lactobacillus strains in step (3) and brewer's yeast s...
Embodiment 3
[0053] Prepare microbial fermented feed according to the following steps:
[0054] (1) Preparation of fermented product: prepare fermented product according to the following fermented product formula,
[0055] High-quality rice bran 60%, rice 32%, glucose 8%;
[0056] (2) Strain preparation: prepare Lactobacillus strains and brewer's yeast strains in a ratio of 10:2;
[0057] (3) Lactobacillus strain rejuvenation: rejuvenate the lactic acid bacteria strain prepared in step (2), the composition of the rejuvenation medium is as follows: casein peptone 10g / L, broth extract 10g / L, yeast extract Compound 5g / L, glucose 20g / L, Tween 1g / L, K 2 HPO 4 2g / L, sodium acetate 5g / L, diammonium citrate 2g / L, MgSO 4 ·7H 2 O 0.2g / L, MnSO 4 ·H 2 O 0.05g / L, the pH value of the culture medium is 6.3, the culture temperature of the culture medium is 38 ℃, and the culture time is 1.5 days;
[0058] (4) Preparation of strain mixture: add 200ml of rejuvenated Lactobacillus strains in step (3)...
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