Method for measuring aromatic amine in plasticine

A determination method and plasticine technology, which are applied to measurement devices, preparation of test samples, instruments, etc., can solve problems such as inappropriate detection of aromatic amines in crayons, and achieve the effect of avoiding matrix interference and being easy to popularize.

Inactive Publication Date: 2011-10-12
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current detection methods for carcinogenic aromatic amines reported in the literature are mainly for samples made of textiles, leather, etc., and because the composition of plasticine is quite different from the above materials, the existing detection methods are not suitable for the detection of aromatic amines in crayons

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  • Method for measuring aromatic amine in plasticine
  • Method for measuring aromatic amine in plasticine
  • Method for measuring aromatic amine in plasticine

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1. Determination method of aromatic amine in plasticine

[0024] (1) Plasticine sample processing

[0025] (1) Take a certain quality of plasticine, cut it into small pieces with a clean blade, and ultrasonically extract the small pieces of plasticine with methanol twice to obtain an extract, and add methanol according to the ratio of 1 gram of plasticine / 40ml of methanol;

[0026] (2) After centrifuging the extract, collect the clarified solution into a heart-shaped bottle, and use a rotary evaporator to evaporate until nearly dry, and then dry it with a slow nitrogen flow;

[0027] (3) Add 200mg / ml sodium dithionite solution (200mg / ml sodium dithionite solution prepared with pH6 citrate buffer solution as solvent) to the chicken heart bottle, add dithionite according to the ratio of 1 gram of plasticine / 0.6 gram of sodium dithionite Sodium disulfite solution, reacted at 70°C for 30 minutes, and cooled to room temperature after the reaction;

[0028] (4) Add a certai...

Embodiment 2

[0058] 1. Determination method of aromatic amine in plasticine

[0059] (1) Plasticine sample processing

[0060] (1) Take a certain quality of plasticine, cut it into small pieces with a clean blade, and extract the small piece of plasticine with acetonitrile ultrasonically twice to obtain an extract, and add acetonitrile according to the ratio of 1 gram of plasticine / 20ml of acetonitrile;

[0061] (2) After centrifuging the extract, collect the clarified solution into a heart-shaped bottle, and use a rotary evaporator to evaporate until nearly dry, and then dry it with a slow nitrogen flow;

[0062] (3) Add 200mg / ml sodium dithionite solution (200mg / ml sodium dithionite solution prepared with pH6 citrate buffer solution as solvent) to the chicken heart bottle, add dithionite according to the ratio of 1 gram of plasticine / 0.4 gram of sodium dithionite Sodium disulfite solution, reacted at 80°C for 20 minutes, and cooled to room temperature after the reaction;

[0063] (4) A...

Embodiment 3

[0075] 1. Determination method of aromatic amine in plasticine

[0076] (1) Plasticine sample processing

[0077] (1) Weigh a certain quality of plasticine, cut it into small pieces with a clean blade, and ultrasonically extract the small pieces of plasticine with isopropanol twice to obtain the extract, add isopropanol according to the ratio of 1 gram of plasticine / 60ml of isopropanol propanol;

[0078] (2) After centrifuging the extract, collect the clarified solution into a heart-shaped bottle, and use a rotary evaporator to evaporate it to nearly dryness, and then blow it dry with a slow nitrogen flow.

[0079] (3) Add 200mg / ml sodium dithionite solution (200mg / ml sodium dithionite solution prepared with pH 6 citrate buffer solution as solvent) into the chicken heart bottle, and add dithionite according to the ratio of 1 gram of plasticine / 1 gram of sodium dithionite. Sodium disulfite solution, reacted at 60°C for 40 minutes, and cooled to room temperature after the reac...

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Abstract

The invention discloses a method for measuring aromatic amine in plasticine, which comprises the following steps of: cutting the plasticine into small pieces, and performing ultrasonic extraction on the small plasticine pieces by using methanol, ethanol, isopropanol or acetonitrile to obtain extracting solution; removing organic solvent in the extracting solution, and adding sodium hyposulfite solution to perform a reduction reaction at the temperature of between 60 and 80 DEG C; after the reaction is finished, regulating the pH value of reaction solution to be between 10 and 12, purifying bya solid phase extraction column, and eluting to obtain eluent; and allowing the eluent to enter a gas chromatograph-mass spectrometer to measure the aromatic amine. The method for measuring the aromatic amine in the plasticine fills the blank of the technology, and the problem of matrix interference caused by other ingredients in the plasticine is solved effectively; and due to the adoption of a common detection device, the method is easy to popularize.

Description

technical field [0001] The invention relates to a method for determining aromatic amines in plasticine. Background technique [0002] Plasticine or children's colored clay currently sold on the market mainly uses water, flour, plastic, dyes, and pigments as raw materials, and also contains a large amount of organic acids, such as benzoic acid. Due to reasons such as unqualified production process and raw materials used, some harmful substances will remain in the plasticine, the most important of which are carcinogenic aromatic amines. This type of substance mainly comes from the decomposition of some azo dyes in plasticine. If these azo dyes are used in plasticine, then when they are in direct contact with the human body, these substances will be metabolized in the human body (such as sweat, saliva) to decompose aromatic amines, which are absorbed by the human body and cause health hazards. In the EN71 series of standards formulated by the European Standardization Committe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N1/34
Inventor 张庆白桦康苏媛吕庆王超
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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