Half-smooth tongue sole hepcidin antimicrobial peptide

A semi-smooth tongue sole and antimicrobial peptide technology, which is applied in the field of semi-smooth tongue sole hepcidin antibacterial peptides, can solve the problems of not easy to produce resistance, no effect of eukaryotic cells, etc., and achieve obvious inhibitory effect

Inactive Publication Date: 2011-10-19
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Unlike antibiotics, the target site of antimicrobial peptides is mainly the cell membrane

Method used

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  • Half-smooth tongue sole hepcidin antimicrobial peptide
  • Half-smooth tongue sole hepcidin antimicrobial peptide
  • Half-smooth tongue sole hepcidin antimicrobial peptide

Examples

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Embodiment Construction

[0021] The method of the present invention will be further described below in conjunction with examples. However, the examples are for illustration only and are not limited thereto. For the experimental methods that do not indicate specific conditions in the following examples, conventional conditions can usually be used, such as the conditions described in the "Molecular Cloning Experiment Guide" written by J. Sambrook (Sambrook), or according to the manufacturer's suggestion conditional run.

[0022] 1. Determination of the antimicrobial peptide sequence of hepcidin from half-smooth tongue sole

[0023] In the present invention, firstly, a cDNA library of tongue sole is constructed, and the antimicrobial peptide hepcidin is cloned from the mixed tissue of tongue sole, and the specific steps are as follows:

[0024] 1. Construction and sequence analysis of cDNA library of tongue sole:

[0025] Extraction of total RNA and synthesis of cDNA: Separate the tissues of the adult...

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Abstract

The invention relates to a half-smooth tongue sole hepcidin antimicrobial peptide with a sequence of SEQ ID NO: 3. Precursor peptide and mature peptide of the half-smooth tongue sole hepcidin antimicrobial peptide are both products that can be obtained through chemical synthesis and genetic recombination expression. Recombination protein obtained through prokaryotic expression of the half-smooth tongue sole hepcidin antimicrobial peptide has an obvious inhibition effect against the growing of staphylococcus aureus, vibrio anguillarum and Edwardsiella tarda. Compared to other antibacterial peptide, the antibacterial peptide provided by the present invention has a more substantial inhibition effect against sea pathogen. Thus, the antibacterial peptide can be used in aqua culture. Therefore,the half-smooth tongue sole hepcidin antimicrobial peptide provided by the present invention can be applied in the preparation of pathogen resisting products, including feed additives, veterinary drugs, antiseptics, and medicine products such as antibiosis medicine.

Description

technical field [0001] The invention belongs to the technical field of antimicrobial peptide screening, and in particular relates to a semi-smooth tongue sole hepcidin antimicrobial peptide. Background technique [0002] Half-smooth tongue sole (English name half-smooth tongue sole, Latin name Cynoglossus semilaevis), is one of the rare and valuable economic fishes in the coastal waters of my country. Because of its fast growth rate and delicious meat, it is deeply loved by consumers. In recent years, the culture of tongue sole has risen rapidly, and its nursery and culture technology has gradually matured and entered the stage of industrialization. However, with the continuous expansion of the breeding scale, the types of diseases, the incidence frequency and their harmfulness are also increasing year by year, which has caused huge economic losses to the entire tongue sole farming industry. At the same time, the extensive application of antibiotics in the mariculture indus...

Claims

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Application Information

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IPC IPC(8): C07K14/46C12N15/12A61P31/04
Inventor 王亚楠张全启刘旭东王旭波齐洁王志刚于海洋王兴莲
Owner OCEAN UNIV OF CHINA
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