Two-path temperature control polymerase chain reactor and real-time detection device

Abstract

The invention relates to a two-path temperature control polymerase chain reactor and a real-time detection device, which are applied in the field of biochemical reaction and medical detection. The two-path temperature control polymerase chain reactor comprises a base layer with a ring-shaped microfluidic channel structure, wherein the base layer is sealed with a cover board layer to form a microfluidic chip, the cover board layer is provided with a sample incoming hole and a sample outgoing hole, a microprocessor is adopted to control two semi-conductor heating and refrigerating devices, a whole microfluidic channel area forms three constant temperature areas, namely a degeneration area, an annealing area and an extension area needed by polymerase chain reaction, the semi-conductor heating and refrigerating devices are arranged under the microfluidic chip, and a platinum resistance temperature sensor manufactured by a semi-conductor process is arranged among the microfluidic chip and the two semi-conductor heating and refrigerating devices, so that temperature feedback signals are provided to the microprocessor, and closed ring control can be formed. The real-time detection systemcomprises a light source, a photoelectric detector and a fluorescence signal acquisition processing system. In the invention, by controlling the two semi-conductor heating and refrigerating devices, the whole microfluidic channel area forms the three constant temperature areas, and the heating and refrigerating device is simplified.

Description

technical field [0001] The invention relates to a fluorescent polymerase chain reaction (Polymerase chain reaction, PCR) on a microfluidic chip to realize DNA amplification and real-time fluorescence detection, which is applied to the fields of biochemical reactions and medical detection. Background technique [0002] Miniaturization is an important development direction in medical and biochemical detection equipment, and the development of micro-electro-mechanical systems (MEMS) technology provides the possibility for PCR reactions to be completed on chips. The traditional fluorescent PCR instrument has the following disadvantages: the instrument is bulky, the heating and cooling rate is slow, the temperature is dynamically controlled, the temperature accuracy is not high, and the long-term temperature sudden change life of the semiconductor heating refrigerator is shortened. There are currently three types of PCR reaction chips manufactured using MEMS technology: micro-wel...

AI Technical Summary

Problems solved by technology

The traditional fluorescent PCR instrument has the following disadvantages: the instrument is bulky, the heating and cooling rate is slow, the temperature is dynamically controlled, the temperature accuracy is not high, and the long-term temperature sudden change of the semiconductor heating refrigerator shortens the service life

The micro-well chip has the following disadvantages: slow heating and cooling rate, dynamic temperature control, and low temperature accuracy. Generally, there are only alloy layer micro-heaters and no active r

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