Two-path temperature control polymerase chain reactor and real-time detection device

Abstract

The invention relates to a two-path temperature control polymerase chain reactor and a real-time detection device, which are applied in the field of biochemical reaction and medical detection. The two-path temperature control polymerase chain reactor comprises a base layer with a ring-shaped microfluidic channel structure, wherein the base layer is sealed with a cover board layer to form a microfluidic chip, the cover board layer is provided with a sample incoming hole and a sample outgoing hole, a microprocessor is adopted to control two semi-conductor heating and refrigerating devices, a whole microfluidic channel area forms three constant temperature areas, namely a degeneration area, an annealing area and an extension area needed by polymerase chain reaction, the semi-conductor heating and refrigerating devices are arranged under the microfluidic chip, and a platinum resistance temperature sensor manufactured by a semi-conductor process is arranged among the microfluidic chip and the two semi-conductor heating and refrigerating devices, so that temperature feedback signals are provided to the microprocessor, and closed ring control can be formed. The real-time detection systemcomprises a light source, a photoelectric detector and a fluorescence signal acquisition processing system. In the invention, by controlling the two semi-conductor heating and refrigerating devices, the whole microfluidic channel area forms the three constant temperature areas, and the heating and refrigerating device is simplified.

Description

technical field [0001] The invention relates to a fluorescent polymerase chain reaction (Polymerase chain reaction, PCR) on a microfluidic chip to realize DNA amplification and real-time fluorescence detection, which is applied to the fields of biochemical reactions and medical detection. Background technique [0002] Miniaturization is an important development direction in medical and biochemical detection equipment, and the development of micro-electro-mechanical systems (MEMS) technology provides the possibility for PCR reactions to be completed on chips. The traditional fluorescent PCR instrument has the following disadvantages: the instrument is bulky, the heating and cooling rate is slow, the temperature is dynamically controlled, the temperature accuracy is not high, and the long-term temperature sudden change life of the semiconductor heating refrigerator is shortened. There are currently three types of PCR reaction chips manufactured using MEMS technology: micro-wel...

AI Technical Summary

Problems solved by technology

The traditional fluorescent PCR instrument has the following disadvantages: the instrument is bulky, the heating and cooling rate is slow, the temperature is dynamically controlled, the temperature accuracy is not high, and the long-term temperature sudden change of the semiconductor heating refrigerator shortens the service life

The micro-well chip has the following disadvantages: slow heating and cooling rate, dynamic temperature control, and low temperature accuracy. Generally, there are only alloy layer micro-heaters and no active refrigerators, such as patents 200310122607.9 and 200510011180.4, etc.

Microfluidic chips have the following disadvantages: Generally, there are only alloy layer micro heaters, and no active coolers, such as patent 200410024703.4, etc.

Micro water bath chip 200610043243.9, etc., with complex heating and cooling structures

The PCR reaction chip manufactured by using MEMS technology solves the problem of large volume of the instrument, but there is also the problem of integrating active cooling and real-time fluorescence detection on the chip

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