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Brown meat essence multi-residue combined detection test paper card and preparation method thereof

A test paper card, clenbuterol technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complicated operation process, limited application scope, difficult popularization and application, etc., to improve work efficiency, ensure food safety, and apply the scope. wide effect

Inactive Publication Date: 2011-11-02
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some of these methods require expensive instruments and equipment, require skilled professionals to operate, the operation process is complicated, and it takes a long time, which limits its application range and is difficult to promote and apply in actual production.
The immunoassay paper method has semi-quantitative and certain quantitative capabilities, and can provide preliminary information on the analyte. This method has high sensitivity and simple analysis process. It has unique advantages in the screening of "clenbuterol", and it needs to be developed first. detection technology, but the detection technology of multi-residue test paper for "Clenbuterol" is still blank in China, and urgently needs to be researched and solved

Method used

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  • Brown meat essence multi-residue combined detection test paper card and preparation method thereof
  • Brown meat essence multi-residue combined detection test paper card and preparation method thereof
  • Brown meat essence multi-residue combined detection test paper card and preparation method thereof

Examples

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preparation example Construction

[0066] 3. Preparation of gold standard binding pad

[0067] Preparation of gold-labeled antibody binding pad:

[0068] Sodium citrate reduction method is an example, first prepare colloidal gold solution: add freshly prepared 1% sodium citrate 8ml to boiling 200ml 0.01~0.02% chloroauric acid solution to obtain a colloidal gold solution with a diameter of about 15nm, and use 0.1mol / L of K 2 CO 3Adjust the pH value to 8.5-9.5, store at 2-8°C for later use; add the monoclonal antibody or polyclonal antibody to be labeled into the gold sol with a pH of 8.5-9.5 at a labeling ratio of 1:2000, label for 10 minutes, and then add 20% PEG10000 to a final concentration of 0.05%, centrifuge at 4°C, 1500-2000rpm for 20min, remove unbound colloidal gold particles, centrifuge at 4°C, 15000rpm for 1h, discard the supernatant, and obtain the initially purified gold-labeled antibody protein, and then use Acryl-dextran S-400 column chromatography for separation and purification to obtai...

Embodiment 1

[0080] Embodiment one: "Clenbuterol" two joint inspection gold standard chromatography test paper card.

[0081] On the basis of obtaining the clenbuterol-carrier protein binding antigen, clenbuterol gold standard and IgG antibody, the test paper card of the present invention is assembled (taking the dual detection of clenbuterol hydrochloride and ractopamine as an example).

[0082] The plastic case of the test paper card includes a base 11 and a panel 10, the base is provided with a fixing groove 12 for installing the test paper core, the panel is provided with a sampling hole 8 and a display window 9, and a T is provided next to the display window. 1 , T 2 and C flags, see Figure 1-4 .

[0083] The structure of the test paper core: the liner 1 is made of hard plastic strips; the sample pad 2 is made of glass fiber cotton; the gold standard binding pad 3 is glass fiber adsorbed with colloidal gold-labeled anti-CL and anti-RAC monoclonal antibodies Cotton; Chromatograp...

Embodiment 2

[0089] Embodiment two: " Clenbuterol ” Gold Standard Chromatographic Test Strip Card for Two Joint Tests (Taking Clenbuterol Hydrochloride and Ractopamine as an Example)

[0090] The structure of the test paper card is basically the same as in Example 1, except that the sample pad is made of nylon membrane; the anti-CL and anti-RAC monoclonal antibodies are replaced by anti-CL and anti-RAC polyclonal antibodies to prepare gold-labeled binding pads; The chromatographic membrane is made of nylon membrane, and the goat anti-rabbit IgG antibody is used to replace the goat anti-mouse IgG antibody to prepare an invisible control line on the chromatographic membrane" ︱ ", the control line is made of pure cellulose film; others such as test sample preparation, operation method and result judgment are the same as in Example 1, and will not be repeated.

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Abstract

The invention discloses a gold-labeled chromatographic detection test paper card for brown meat essence multi-residue analysis, and the test paper card comprises a plastic support and a test paper core, wherein the test paper core is formed by sequentially bonding a sample pad, a gold-labeled material combination pad, a chromatographic film and a water absorption pad onto a lining board; the gold-labeled material combination pad is glass fiber cotton adsorbed with 2-5 mixed gold-labeled antibodies; the gold-labeled antibodies are colloidal-gold-labeled monoclonal antibodies or polyclonal antibodies for resisting brown meat essence; detection lines are 2-5 corresponding brown meat essence-carrier protein conjugated antigen strips; control lines are formed by printing of goat or rabbit anti-mouse IgG, goat anti-rabbit IgG or rabbit anti-carrier protein IgG antibodies; and the detection lines and the control lines are arranged in parallel in the transverse direction along the chromatographic film. The test paper card has high specificity and sensitivity, and the minimum detection limit can be 1ppb, thereby realizing fast detection of brown meat essence multi-residue gold-labeled chromatography. By using the test paper card, multiple brown meat essence residues can be simultaneously detected after one-step sample application, thereby greatly saving the detection time, improving the working efficiency and saving the detection cost.

Description

technical field [0001] The invention relates to a device for rapidly detecting trace amounts of "clenbuterol", in particular to a gold standard chromatography detection test paper card for multi-residue analysis of "clenbuterol" and a preparation method thereof. Background technique [0002] "Clenbuterol" refers to a class of drugs, any substance that can promote lean growth and reduce fat deposition can be called "Clenbuterol", including "Clenbuterol" (Clenbuterol, CL), Clenbuterol hydrochloride Its hydrochloride, "ractopamine" (Ractopamine, RAC, also translated as retopamine), "salbutamol" (Salbutamol, SAL), "terbutaline" (Terbutaline, TBL) and "cimaterol "(Cimaterol, CIM) et al. "Clenbuterol" has the effect of promoting muscle development and lipolysis when the dose in the production of meat animals is 5-10 times that of the therapeutic dose, and can increase the lean meat percentage of meat animal carcasses. Because consumers have a special preference for lean meat, pi...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/531G01N33/566
Inventor 职爱民胡骁飞张改平邓瑞广李孟顺杨继飞刘庆堂赵东邢广旭柴书军杨艳艳李青梅滕蔓
Owner HENAN ACAD OF AGRI SCI
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