Fluorescence thin layer identification method for bighead atractylodes rhizome
A technology of thin-layer identification and fluorescence enhancement, which is applied in the field of fluorescence-increasing thin-layer identification of Atractylodes macrocephala, can solve the problems of endangering the health of patients, low renewal rate of thin-layer identification, delaying patients' condition, etc., and achieve the effect of increasing the renewal rate
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Embodiment 1
[0068] Thin-layer identification of toad venom by fluorescence detection:
[0069] a. Take 0.05 g of toad venom medicinal material and toad venom reference medicinal material powder respectively, add 3 milliliters of methanol, sonicate for 15 minutes, and take the supernatant as the test solution and the reference solution;
[0070] b. Take 2 microliters each of the test solution and the reference solution, and place them on the same GF respectively 254 On the thin-layer plate, develop with cyclohexane-ethyl acetate-formic acid with a volume ratio of 4:6:0.2 as the developer, take it out, dry it, and inspect and take pictures under 254nm and 365nm ultraviolet lamps respectively;
[0071] c. Spray 10% sulfuric acid ethanol solution as a fluorescence enhancer on the thin-layer plate, and heat at 105°C until the spots are clearly colored;
[0072] d. Check and take pictures under 365nm ultraviolet light;
[0073] see results figure 1 , it can be seen that after the fluorescen...
Embodiment 2
[0075] TLC identification of Atractylodes macrocephala by fluorescence detection:
[0076] a. Take 0.5 g of Atractylodes macrocephala medicinal material and Atractylodes macrocephala reference medicinal material powder respectively, add 5 milliliters of methanol, sonicate for 10 minutes, and take the supernatant as the test solution and the reference solution;
[0077] b. Take 4 microliters each of the test solution and the reference solution, and place them on the same GF respectively. 254 On the thin-layer plate, develop with cyclohexane-ethyl acetate-formic acid with a volume ratio of 8:2:0.2 as the developer, take it out, dry it, and inspect and take pictures under 254nm and 365nm ultraviolet lamps respectively;
[0078] c. Spray 10% sulfuric acid ethanol solution as a fluorescence enhancer on the thin-layer plate, and heat at 105°C until the spots are clearly colored;
[0079] d. Check and take pictures under 365nm ultraviolet light;
[0080] see results figure 2 It c...
Embodiment 3
[0082] TLC identification of forsythia by fluorescence detection:
[0083] a. Take 0.4 g of Forsythia medicinal material and Forsythia reference drug respectively, add 5 milliliters of methanol, ultrasonically treat for 10 minutes, and take the supernatant as the test solution and the reference solution;
[0084] b. Take 4 microliters each of the test solution and the reference solution, and place them on the same GF respectively. 254 On the thin-layer plate, develop with cyclohexane-ethyl acetate-formic acid with a volume ratio of 10:2:0.2 as the developer, take it out, dry it, and inspect and take pictures under 254nm and 365nm ultraviolet lamps respectively;
[0085] c. Spray 10% sulfuric acid ethanol solution as a fluorescence enhancer on the thin-layer plate, and heat at 105°C until the spots are clearly colored;
[0086] d. Check and take pictures under 365nm ultraviolet light;
[0087] see results image 3 , it can be seen that after the fluorescence is increased, th...
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