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Method and special test paper for estrogen detection

An estrogen and test strip technology, applied in the field of estrogen detection, can solve the problems that colloidal gold immunochromatography is difficult to meet the requirements of sensitivity and quantification, cannot perform accurate quantification, and the sensitivity is not high enough, and achieves simple and convenient detection time and high sensitivity. , the effect of improving sensitivity

Active Publication Date: 2011-11-23
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although immunochromatography has such attractive advantages, its application is also limited, mainly because the main marker used in immunochromatography is colloidal gold, and the results are generally judged by naked eyes, so the sensitivity is not high enough , can not be accurately quantified, only semi-quantitative determination
Therefore, for the determination of estrogen with very low content in the environment and agricultural products, it is difficult for ordinary colloidal gold immunochromatography to meet the sensitivity and quantitative requirements.

Method used

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  • Method and special test paper for estrogen detection
  • Method and special test paper for estrogen detection
  • Method and special test paper for estrogen detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the test paper that detects estradiol

[0044] Method I

[0045] 1. The composition of the test paper for detecting estradiol

[0046] Test strips without marker pads ( figure 1 ).

[0047] The test paper is composed of a sample absorbent pad 1, a reaction film 2, a water absorbent pad 3 and a bottom plate 4;

[0048] The sample absorbent pad, the reaction film and the water absorbent pad are pasted on the bottom plate in sequence, the end of the sample absorbent pad is connected to the beginning of the reaction film, the end of the reaction film is connected to the beginning of the water absorbent pad, and the beginning of the sample absorbent pad Align with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0049] There is a detection zone 5 and a quality control zone 6 on the reaction film, both of which are dot-shaped; the detection zone is located near the end of the sample absorbent ...

Embodiment 2

[0124] Embodiment 2, the test paper that detects estrone

[0125] Method I

[0126] 1. The composition of the test paper for detecting estrone

[0127] Test strips without marker pads ( figure 1 ).

[0128] Except that the conjugate of estrogen and carrier protein is the conjugate of estrone and ovalbumin, the other structures are the same as step 1 of method I in Example 1.

[0129] 2. Preparation of test paper

[0130] The preparation method of the test paper without marker pad mainly comprises the steps:

[0131] 1) Prepare a reaction membrane containing a detection area coated with a conjugate of estrone and ovalbumin and a quality control area coated with a quality control antigen (rabbit IgG);

[0132] 2) Preparation of fluorescent labeling mixture

[0133] Mix Cy3-labeled anti-estrone antibody and Cy3-labeled goat anti-rabbit antibody at a volume ratio of 1:1 to obtain a fluorescent labeling mixture;

[0134] 3) Assemble the sample absorbent pad, reaction membran...

Embodiment 3

[0196] Embodiment 3, detect the test paper of diethylstilbestrol

[0197] Method I

[0198] 1. The composition of the test paper for detecting diethylstilbestrol

[0199] Test strips without marker pads ( figure 1 ).

[0200] Except that the conjugate of estrogen and carrier protein is a conjugate of diethylstilbestrol and ovalbumin, the other structures are the same as step 1 of method I in Example 1.

[0201] 2. Preparation of test paper

[0202] The preparation method of the test paper without marker pad mainly comprises the steps:

[0203] 1) Prepare a reaction membrane containing a detection area coated with a conjugate of diethylstilbestrol and ovalbumin and a quality control area coated with a quality control antigen (rabbit IgG);

[0204] 2) Preparation of fluorescent labeling mixture

[0205] Mix Alexa Fluor647-labeled anti-dethylstilbestrol antibody and Alexa Fluor647-labeled goat anti-rabbit antibody according to a certain ratio to obtain a fluorescent labelin...

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Abstract

The invention discloses a method and a special test paper for estrogen detection. The test paper for estrogen detection provided by the invention is a test paper obtained through step 1 or step 2 described below: 1, a test paper comprises a sample absorption pad, a reaction film, and a water absorption pad which are connected in sequence, wherein the reaction film comprises a detection zone which is coated with a conjugate of estrogen and carrier proteins and a quality control zone which is coated with quality control antigens; and 2, a test paper comprises a sample absorption pad, a maker pad, a reaction film, and a water absorption pad which are connected in sequence, wherein the tag pad is coated with fluorescein-labeled estrogen antibodies and fluorescein-labeled quality control antibodies, and the reaction film comprises a detection zone which is coated with a conjugate of estrogen and carrier proteins and a quality control zone which is coated with quality control antigens. The test paper for estrogen detection of the present invention has the advantages of high sensitivity, accurate quantification, strong specificity, simplicity and convenience, and short detection time.

Description

technical field [0001] The invention relates to a method for detecting estrogen and special test paper thereof. Background technique [0002] Natural estrogens mainly include estradiol, estrone, and estriol, while synthetic estrogens mainly include diethylstilbestrol, dienestrol, hexestrol, etc., which are mainly used as oral contraceptives and anabolic hormones for livestock growth in agriculture , by enhancing appetite, suppressing estrus, improving feed conversion rate and controlling sex, etc., it can achieve the purpose of greatly improving the economic benefits of animal breeding, resulting in an upward trend in estrogen pollution residues in agricultural products. Estrogen in agricultural products can cause a variety of diseases to animals and humans by disrupting the normal functions of endocrine, immune, and nervous systems. , ovarian cancer, breast cancer and other diseases. Men often suffer from symptoms such as testicular cancer, prostate cancer, and decreased ...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/558
Inventor 陈爱亮杨曙明陈刚于洪侠邱静张妍
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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