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Method for ultrasonic-assisted extraction of total flavones in cassia seed serving as Chinese medicine

A technology of total flavonoids and cassia seeds, which is applied in medical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of limiting the application of flavonoids, low extraction yield, and low extraction yield of total flavonoids. Achieve the effects of promoting dissolution and diffusion, reducing production costs and improving production efficiency

Inactive Publication Date: 2013-07-10
乔德亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of these two extraction methods is that the extraction yields of total flavonoids in Semen Cassiae are all low, only 1.01% and 2.03%.
The low extraction yield limits the application of flavonoids in Semen Cassiae

Method used

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  • Method for ultrasonic-assisted extraction of total flavones in cassia seed serving as Chinese medicine
  • Method for ultrasonic-assisted extraction of total flavones in cassia seed serving as Chinese medicine
  • Method for ultrasonic-assisted extraction of total flavones in cassia seed serving as Chinese medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0024] In the present embodiment, the method of ultrasonic-assisted extraction of total flavonoids in cassia seeds is operated according to the following steps:

[0025] a. The cassia seeds are dried at 60°C and pulverized to obtain powder;

[0026] b. The powder obtained in step a is wrapped with filter paper and tied with cotton thread, placed in a Soxhlet extractor, added with petroleum ether, degreased by refluxing in a water bath at 75°C, and when the petroleum ether extract changed from pale yellow to colorless , take out the filter paper bag, and evaporate the petroleum ether in the sample to obtain degreasing powder;

[0027] c, add ethanol to the degreasing powder obtained in step b, put the ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., Shumei brand KQ-500B type, ultrasonic power 500W) to carry out leaching, extraction temperature 70 ℃, extraction time 60min, the ratio of liquid to material is 50mL / g, the volume percent concentration of ethanol is 70%,...

Embodiment 2

[0035] This example is an optimization experiment of the extraction conditions of ultrasonic-assisted extraction of total flavonoids in cassia seeds.

[0036] 1. A single factor experiment on the optimization of conditions for ultrasonic-assisted extraction of total flavonoids from cassia seeds

[0037] ①Influence of extraction temperature on the extraction yield of total flavonoids from Cassia

[0038]The steps of the extraction method are the same as in Example 1. The extraction temperature is set at five gradients of 30°C, 40°C, 50°C, 60°C and 70°C. The ultrasonic power, extraction time, liquid-to-solid ratio and volume percent concentration of ethanol are respectively 500W, 40min, 30mL / g and 70%. See the following table for the extraction yield of total flavonoids from Semen Cassiae under different extraction temperature conditions:

[0039] Table 1

[0040]

[0041] It can be seen from Table 1 that the extraction yield of total flavonoids is higher when the extract...

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Abstract

The invention discloses a method for the ultrasonic-assisted extraction of total flavones in cassia seed. The method comprises the following steps of: drying the cassia seed serving as a raw material, crushing, defatting by using petroleum ether, performing ultrasonic-assisted leaching in ethanol, centrifuging leaching liquor, leaving supernate, concentrating the supernate under reduced pressure to form paste, drying and crushing to obtain cassia seed total flavon extract. The method has the advantages of mild extraction condition, simple process, high yield of total flavone extraction and the like, so the production efficiency can be improved, and the production cost can be reduced.

Description

1. Technical field [0001] The invention relates to a method for ultrasonic-assisted extraction of total flavonoids in cassia seeds. 2. Background technology [0002] Cassia seeds are the dried and mature seeds of the leguminous plant Cassia (Cassia obtusifolia L.) or small Cassia (Cassia tora L.), which is one of the traditional Chinese medicine varieties included in the "Pharmacopoeia of the People's Republic of China" (2010 Edition), and also It is one of the medicinal and edible homologous plants announced by the Ministry of Health (Weifa Jianfa [2002] No. 51). As a clinical traditional Chinese medicine, cassia seed was first recorded in the "Shen Nong's Materia Medica", and it was listed as a top-grade medicine. It was named after the effect of improving eyesight. Eyesight, laxative effect. Modern research shows that cassia seeds contain anthraquinones, polysaccharides, flavonoids, fatty acids, amino acids and inorganic elements and other chemical components, which hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/482A61K131/00
Inventor 乔德亮朱明贵
Owner 乔德亮