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Method for synthesizing isoquercitrin by metal ion-reinforced enzyme method

A technology for isoquercitrin and enzymatic synthesis, which is applied in the field of metal ion-enhanced enzymatic synthesis of isoquercitrin, can solve the problems of difficult preparation of isoquercitrin, high hydrolysis temperature, equipment corrosion, etc., and overcome the efficiency of catalytic synthesis Low, enhanced synthesis efficiency, no environmental pollution effect

Inactive Publication Date: 2013-11-06
JIANGSU UNIV OF SCI & TECH
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] Patent CN 101985639A introduces that the preparation methods of isoquercitrin mainly include extraction method and hydrolysis method: (1) the distribution of isoquercitrin in nature is less, and its content is only tens of thousands or even one hundred thousand It is difficult to prepare isoquercitrin directly from natural plants; (2) the hydrolysis temperature of the acid-catalyzed hydrolysis method is high, the acid used seriously corrodes the equipment, and produces a large amount of acidic wastewater, and the hydrolysis of rutin During the process, quercetin and isoquercitrin coexist, and the hydrolyzate must be separated to obtain isoquercitrin (African Journal of Biotechnology, 2011, 10(8): 1460-1466)

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  • Method for synthesizing isoquercitrin by metal ion-reinforced enzyme method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] With 0.01mol / L disodium hydrogen phosphate-0.01mol / L citric acid buffer solution, prepare 0.01g / L rutin mother solution with pH=5. Add 0.1g / L α-L-rhamnosidase solution to the rutin mother liquor by 1% by volume, add CoCl 2 to Co 2+ The concentration was 0.1 mmol / L, and the reaction was shaken at 50 r / min for 1 h at 10°C. After the reaction, the conversion rate of the product isoquercitrin detected by HPLC was calculated to increase by 0.1% relative enzyme activity.

Embodiment 2

[0028] Use 1mol / L disodium hydrogen phosphate-citric acid buffer solution to prepare 1 g / L rutin mother solution with pH=9. Add 100g / L α-L-rhamnosidase solution in the rutin mother liquor by volume ratio 50%, add Na 2 SO 4 to Na + The concentration was 100mmol / L, and the reaction was shaken at 60°C and 200r / min for 48h. After the reaction, the conversion rate of the product isoquercitrin detected by HPLC was calculated to increase by 0.1% relative enzyme activity.

Embodiment 3

[0030] With 0.2mol / L disodium hydrogen phosphate-0.1mol / L citric acid buffer solution, prepare 0.3g / L rutin mother solution with pH=7. Add 10g / L α-L-rhamnosidase solution in the rutin mother liquor by volume ratio 5%, add AlCl 3 to Al 3+ The concentration was 1.0mmol / L, and the reaction was shaken at 120r / min at 40°C for 20h. After the reaction, the conversion rate of the product isoquercitrin detected by HPLC was calculated to increase by 8% relative to the enzyme activity.

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Abstract

A method for synthesizing isoquercitrin by a metal ion-reinforced enzyme method comprises the step of adding metal ions into an enzymatic isoquercitrin synthesis system in order to increase the efficiency of synthesization. The method is easy to operate, reaction conditions are mild, the product yield is high, the method is environment-friendly, and overcomes the defect of conventional glycosidase-catalysed synthesis, i.e. low efficiency, and high-purity isoquercitrin can be easily produced industrially.

Description

technical field [0001] The invention belongs to the field of enzymatically synthesizing food additives, in particular to a metal ion-enhanced enzymatically synthesizing method for isoquercitrin. Background technique [0002] Prior art: Isoquercitrin (Isoquercitrin), that is, quercetin-3-O-β-D-glucoside (Quercetin-3-glucose), is a very rare in nature with significant antioxidant, Anti-tumor and other biologically active flavonoids are also high-grade food additives, auxiliary drugs or active ingredients of drugs developed by major international pharmaceutical and food companies. [0003] Patent CN 101985639A introduces that the preparation methods of isoquercitrin mainly include extraction method and hydrolysis method: (1) isoquercitrin is rarely distributed in nature, and its content is only tens of thousands or even hundreds of thousands It is difficult to prepare isoquercitrin directly from natural plants; (2) the hydrolysis temperature of the acid-catalyzed hydrolysis me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/60
Inventor 王俊吴福安张旭东夏睿
Owner JIANGSU UNIV OF SCI & TECH