Application of 17a alpha-D-homo-ethynylestradiol-3-ethyl ester in preparation of medicines for treating radiation induced aplasticanemia
A technology for aplastic anemia and high ethinyl estradiol, which is applied in drug combinations, blood diseases, and pharmaceutical formulations, and can solve problems such as large side effects, high cost of long-term immunosuppressive agents, and difficulty in matching bone marrow transplantation.
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[0026] Purpose
[0027] Observation of the therapeutic effect of E838 on the peripheral blood of aplastic mice
[0028] Experimental Materials
[0029] 1. Experimental animals: BALB / C mice, DBA / 2 mice, male, provided by the Institute of Experimental Animals, Chinese Academy of Medical Sciences (certificate number: SCXK (Beijing) 2005-0013). The feed was clean-grade full-price mouse feed provided by Beijing Keao Xieli Feed Co., Ltd.
[0030] 2. Experimental equipment: Tianjin Institute of Physics and Technology 60Co source γ-rays, the dose is 5.0Gy, and the dose rate is 1.1Gy / min. Automatic blood analyzer (pocH-100i, Sysmex, Japan), paraffin slicer for paraffin blocks (LM2235, Leica, Germany), upright microscope (BX51, Olympus, Japan), camera (D90, Nikon, Japan).
[0031] 3. Reagent: E838 was developed by our institute (see ZL200510016408.9), and injection oil was provided by Tianjin Amino Acid Company. RPMI-1640 medium was purchased from SIGMA Company.
[0032] expe...
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