Freezing storage liquid for freezing and storing mononuclear cells
Patent Information
- Authority / Receiving Office
- CN ยท China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHENZHEN BEIKE BIOTECH
- Publication Date
- 2012-01-04
- Estimated Expiration
- Not applicable ยท inactive patent
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Abstract
Description
technical field
[0001] The invention belongs to the field of biotechnology, and in particular relates to a cryopreservation solution for cryopreserving mononuclear cells and a preparation method thereof. Background technique
[0002] Normally, liquids freeze to form regular or irregular crystals that can rupture cell membranes and cause direct damage to living cells. Therefore, in order to reduce cell damage during cryopreservation, it is often necessary to use a cryopreservation solution containing a cryoprotectant. Commonly used cryoprotectants include dimethyl sulfoxide (DMSO), glycerin, and dextran, among which DMSO is the most commonly used. The use of pure animal serum combined with DMSO is a commonly used cryopreservation solution, but animal serum may contain animal pathogenic microorganisms, which may cause cross-infection with frozen cells. Once infection occurs, subsequent resuscitation and washing are difficult to remove. Culture medium and hydroxyethyl starch ...