Monoclonal antibody against riemerella anatipestifer (RA)
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A technology of Riemerella anatipestifer and Bacillus is applied in the field of antibody engineering and double-antibody sandwich ELISA method to detect Riemerella anatipestifer, which can solve complex problems and achieve the effect of sensitive detection method and wide application value
Inactive Publication Date: 2012-01-04
YANGZHOU UNIV
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Hu Qinghai et al. used lipopolysaccharide (LPS) as the coating antigen, established an indirect ELISA method to detect antibodies in serum, and the ducks artificially infected with RA could detect antibodies within 72 hours of infection; however, due to the complexity of RA serotypes, this m
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[0011] Biological material in the present invention can be obtained through the following ways
[0012] 1, 2, 3, 11 four serotypes of RA: References 1, 3 types: Yan Tianjiu. Epidemiological investigation and control of Riemerella anatipestifer in Baise City [D]. Guangxi University, 2007. Type 2 and Type 11: Yang Yong. Isolation, Identification and Pathogenicity of Riemerella anatipestifer in Some Areas of Jiangsu[D], Yangzhou University, Professional Master Thesis, 2008; Yun Shuili, Yang Yong, Wang Xiaobo, etc. 11 Isolation, identification and pathogenicity of Riemerella anatipestifer type [J]. Journal of Preventive Veterinary Medicine, 2009, 8:605-609.
[0013] Pasteurella (domestic standard virulent strain C48-1, purchased from China Veterinary Drug Control Institute).
[0015] Escherichia coli (DH5α, purchased from Promega).
[0016] 1. Preparation of monoclonal antibody
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Abstract
The invention belongs to the field of biotechnology, specifically relates to a monoclonal antibody against riemerella anatipestifer (RA). The preservation number of the monoclonal antibody 5G7 is CGMCC No. 5165. With adopting the monoclonal antibody to prepare the immune colloidal gold strip for detecting the RA, the pathogen of the RA can be directly detected, and the RA strains such as serotypes of 1, 2, 3 and 11 can be synchronously detected, such that the monoclonal antibody against the RA has a wide application value.
Description
technical field [0001] The invention belongs to the field of biotechnology and relates to antibody engineering technology, specifically monoclonal antibodies for recognizing different serotypes of Riemerella anatipestifer and a double-antibody sandwich ELISA method based on this to detect Riemerella anatipestifer bacilli. Background technique [0002] Riemerella anatipestifer (RA) is the pathogenic bacterium that causes infectious serositis in ducks. It mainly infects a variety of poultry, mainly waterfowl, and causes acute or chronic infectious diseases. The diseased ducks often show lethargy, crooked neck, lameness, yellow-green loose feces, serous secretions from the eyes and nose, and then neurological symptoms such as convulsions and opisthotonos. The lesions are characterized by fibrinous pericarditis, perihepatitis, air sacculitis, caseous salpingitis and meningitis. At present, serum types 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 13, 14 and 4 unnamed types have been reporte...
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