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Method for separating and extracting enramycin by using macroporous weakly-acidic cationic resin

A technology of cationic resin and enramycin, which is applied in the field of separation, can solve the problems of difficulty in meeting the accuracy requirements of content detection, easy loss, and slow development of drug resistance.

Inactive Publication Date: 2013-06-05
SICHUAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF THE PEOPLES REPUBLIC OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sensitive bacteria hardly develop resistance to enramycin, and under experimental conditions, drug resistance is also very slow, even if it does appear, drug resistance is unstable and easily lost
3. In terms of drug residue research, enramycin is extremely difficult to be absorbed when taken orally, and the drug is mainly excreted through feces
However, the inventive method can only control the yield of enramycin in the mycelium at 60% to 71%, the recovery rate is low, the consumption is too much for large-scale industrial production, and the content detection is also difficult to meet the accuracy requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Separation and purification of enramycin with HD-2 type cationic resin

[0039] This example is the conditions used on the transferred macroporous adsorption resin.

[0040] 1. Mix 10 g of crude enramycin + 50 ml of 50% methanol water (pH 3.0), stir, filter, and take the supernatant as the extract for later use.

[0041] 2. Separation and purification, this embodiment is the condition used on the macroporous adsorption resin used.

[0042] (1) Resin pretreatment:

[0043] Immerse HD-2 macroporous weakly acidic cationic resin in sufficient amount of methanol, stir slowly to make it fully mixed, after standing, carefully remove the upper layer of methanol liquid, add enough water, stir and mix, set aside for later use.

[0044] (2) Column processing:

[0045] Choose a chromatographic column with a size of 1cmX20cm, pack the pretreated resin into the column, and keep the packed column from drying out during the entire column packing process. Use 25ml of pH 8....

Embodiment 2

[0048] Embodiment Two Use the method of the present invention to separate and purify enramycin with macroporous weakly acidic cationic resin HD-2

[0049] 1. Rough extraction of samples

[0050] Enramycin crude product 130g+200ml (methanol)+200mlH 2 o

[0051] Adjust the pH to 3.0 with concentrated hydrochloric acid HCl, stir well for 120 minutes, let stand, filter, and take the supernatant as the extract for later use.

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PUM

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Abstract

The invention provides an effective method for separation and purification of enramycin, and particularly provides a method for separating and extracting enramycin by using macroporous weakly-acidic cationic resin. The method comprises the following steps: completely activating the macroporous weakly-acidic cationic resin by using methanol, and then adding sufficient water in the activated resin for stirring and mixing; carrying out column packing, and then passing a weak-alkaline methanol solution through the column for balancing; heating enramycin mycelium in a water bath by using ethanol, and then refluxing so as to obtain a crude product; mixing the obtained crude product with a weakly-acidic methanol water solution; filtering, and adjusting the pH of the obtained solution to alkalescence; loading the column, adsorbing, and then washing with methanol water to remove impurities; and eluting with a proper amount of methanol water, collecting eluent, concentrating, and drying by distillation so as to obtain the product enramycin. The method provided by the invention is simple in process, strong in maneuverability, and high in recovery rate and purity.

Description

technical field [0001] The invention belongs to the technical field of separation, and relates to a method for separating and extracting enramycin by using a macroporous weakly acidic cationic resin. Background technique [0002] Since the invention of antibiotics, they not only have remarkable effects in the treatment and prevention of human diseases, but also play a huge role in animal husbandry feed additives. The growth-promoting effect of antibiotics on animals was discovered in the late 1940s by Stocktadt et al. Later, it was found that antibiotics such as tetracyclines, macrolides, and β-endocylamines were added to feed, all of which could promote growth and increase growth. weight, increase production, and improve feed remuneration. In 1950, the US FDA officially approved the addition of antibiotics to feed. Antibiotics have positive and stable effects on the growth and production performance of livestock and poultry. The livestock and poultry industries in many co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/64C07K1/18
Inventor 严玉宝谢晶余华叶健强胡娟廖党金周岷江李江凌曹冶李红赵素君文豪
Owner SICHUAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF THE PEOPLES REPUBLIC OF CHINA