Method for largely and rapidly reproducing lactic acid bacteria

A lactic acid bacteria, rapid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of complex production process, high production cost, low yield, etc., and achieve fast reproduction and production, low cost, and fatality rate Reduced effect

Active Publication Date: 2012-01-11
黄家总
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the requirements for lactic acid bacteria production equipment are high, the production process is

Method used

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Embodiment Construction

[0018] A kind of rapid breeding method for producing lactic acid bacteria described in the embodiment of the present invention comprises the following steps:

[0019] 1) Collect the stems and leaves of the top of the natural growing wormwood or the tea stem with a length of about 10-20cm or the leaves at the top of the tea stem, and mix the wormwood or tea, molasses, and salt in a weight fraction ratio of 16:8:2;

[0020] 2) Put the mixed wormwood or tea leaves, molasses, and salt into a plastic film bag, tie the mouth of the plastic film bag tightly, and ferment naturally at room temperature;

[0021] 3) Take 60kg of molasses and 1kg of salt, add 300L of water and stir to dissolve, pump them into a plastic tank with a capacity of 3000L, and add water to 3000L. The pump is arranged inside the plastic tank, the pump is connected to the inlet of the ultra-fine equipment, the outlet pipe of the ultra-fine equipment extends into the plastic tank, and the solution in the plastic ta...

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Abstract

The invention relates to a method for largely and rapidly reproducing lactic acid bacteria, which comprises the following steps: (1) mixing wormwood or tea-leaf, molasses with common salt in the proportion of 16:8:2 in percentage by weight; (2) filling the mixed wormwood or tea-leaf, molasses and common salt into a plastic film bag, tightening the mouth of the plastic film bag, and spontaneously fermenting at normal temperature; (3) fetching 60kg of molasses and 1kg of common salt and adding 300L of water to stir and dissolve the molasses and the common salt, and then, adding the water into a plastic tank of which the capacity is 3000L to 3000L to prepare a nutrient solution for reproducing the lactic acid bacteria through the suction of a pump; and (4) after 7 days of spontaneous fermentation, taking out a fermentation solution, i.e. a lactic acid bacteria strain stock solution, adding 100L of lactic acid bacteria strain stock solution into the plastic tank, and then adding the nutrient solution of the lactic acid bacteria to 3000L. The invention has the advantages as follows: the technology and the method for largely and rapidly reproducing the lactic acid bacteria by fermenting the wormwood or the tea-leaf are at least 100 times higher than a traditional lactic acid bacteria production technology in speed, and the cost price is only 1/100 the cost price of the traditional lactic acid bacteria production technology.

Description

technical field [0001] The invention relates to a method for rapidly multiplying and producing lactic acid bacteria in large quantities. Background technique [0002] Lactic acid bacteria refers to the general term for a class of non-spore-forming, Gram-positive bacteria whose main product is lactic acid. All bacteria that can produce lactic acid from the fermentation of glucose or lactose are collectively called lactic acid bacteria. This is a rather complex group of bacteria, which can be divided into at least 18 genera and more than 200 species. Except for a few, most of them are essential flora in the human body and have important physiological functions, which widely exist in the intestinal tract of the human body. At present, it has been confirmed by biologists at home and abroad that there is a very close direct relationship between intestinal lactic acid bacteria and health and longevity. Lactic acid bacteria are a type of probiotic bacteria that exist in the huma...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
Inventor 黄家总
Owner 黄家总
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