New high-quality seed reserving method of spring cabbage
A technology of cabbage and germplasm, applied in botany equipment and methods, seed and rhizome treatment, horticulture, etc.
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Embodiment 1
[0026] Embodiment 1. Determination of various parameters in the method of the present invention.
[0027] The inventor of the present invention first thought of and tried a new spring cabbage seed strain preservation strategy in 2006, which is the same as the solution strategy provided by the present invention: "healthy bulb selection-dehydration treatment-refrigeration-pre-transplanting treatment "Four key steps, and the parameters of each step were further determined in the following three consecutive years. The comparison test shows that the preservation and survival rate of the seed plants is obviously superior to the axillary bud cutting method and the tissue culture propagation method of the prior art at that time.
[0028] Steps:
[0029] (1) Pre-treatment of the selected seed strains in a low-temperature cabinet for refrigeration
[0030] Dig up the selected spring cabbage excellent germplasm, shake off the root soil gently; remove the outer leaves, but be careful n...
Embodiment 2
[0044] Embodiment 2 comparative test
[0045] The treatment steps of the low-temperature refrigeration method in this experiment are the same as those in Table 1 of Example 1. The dehydration treatment time is 7 days, the refrigeration temperature is 2-5° C., and the water content of the sand bed is such that the sand can be grasped into balls without dripping water.
[0046] The axillary bud cutting method refers to the records of Li Shuxuan et al. ("Cabbage and Cabbage Cutting Rice Law Enforcement", Plant Physiology Communication, 1980, 1: 41-44): after the leaf balls are cut off, the axillary buds grow, and when the axillary buds grow to 5- 6 leaves, cut off for cuttings.
[0047] The method of tissue culture expansion refers to the records of Zhang Zhiqiang et al.
[0048] The results are shown in Tables 3-4 below.
[0049] Table 3 Effects of axillary bud cutting method and freezer method on the survival rate of spring cabbage plants
[0050]
[0051] Table 4. Compar...
Embodiment 3
[0055] Adopting exactly the same operation steps as in Example 2, after multi-generation breeding of the excellent cabbage variety 88-62 introduced from the Netherlands in 1988, an excellent spring cabbage inbred line 88-62-1-1 was obtained. The color of the ball is greener, the shape of the ball is nearly round, and the resistance to cracking is strong. Using 88-62-1-1 as the male parent and the female parent CMS87-534, an excellent cabbage hybrid 'Zhonggan 192' was prepared (Zhuang Mu et al. "A New Spring Cabbage Variety 'Zhonggan 192'", Acta Horticultural Science 2010, 37( 11): 1881-1882).
[0056] In addition, during the implementation of the technical solution of the present invention, there are also the following precautions:
[0057] 1. When selecting reserved seed plants, try to choose seed plants with tightly packed balls, no disease in the roots and leaf balls, and few or no ball worm eyes. Be careful not to damage the shortened stems when removing the outer leaves....
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