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Method for extracting onion enzyme from fistular onion stalk

A scallion and extract technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., to achieve the effect of stable process quality and easy operation

Inactive Publication Date: 2012-01-18
张介眉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And how to obtain the onion enzyme from the white of the scallion without any literature report

Method used

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  • Method for extracting onion enzyme from fistular onion stalk

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Experimental program
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Effect test

Embodiment 1

[0019] (1) Keep the scallions at a temperature of -4°C to 4°C for 12 hours to 24 hours;

[0020] (2) Add pH 6.5 Na to 350 grams of cooled scallion 2 HPO 4 and KH 2 PO 4 Mix 500 ml of phosphate buffer, 45 ml of glycerin, and 0.5 g of disodium ethylenediaminetetraacetic acid (EDTA), pre-cool at -4°C to 4°C for 12 hours, then add 0.001 g of 5'-pyridoxal phosphate; After homogenization at -4°C to 4°C; centrifuge at 0°C to 5°C at a speed of 6000 rpm to 8000 rpm for 20 minutes to 30 minutes to obtain the first solid phase and the first liquid phase; 500 ml of Na 2 HPO 4 and KH 2 PO 4 Phosphate buffer contains Na 2 HPO 4 3.5 g, with KH 2 PO 4 1.5 grams.

[0021] (3) Add 15% polyethylene glycol 8000 of the mass of the first liquid phase to the first liquid phase, and let it stand after stirring; at 0°C to 5°C, the rotating speed is 6000 rpm to 8000 rpm, and the time is 20 Minutes to 30 minutes for centrifugation to obtain protein precipitates and supernatant;

[0022] (4...

Embodiment 2

[0025] According to the above process steps

[0026] In step 2, raw materials: 350 grams of scallion white, 500 ml of Na at pH 6.5 2 HPO 4 and KH 2 PO 4 Phosphate buffer contains Na 2 HPO 4 6 grams, with KH 2 PO 4 4 g; 60 ml of glycerin, 2 g of disodium edetate, 0.005 g of pyridoxal 5'-phosphate

[0027] 25% polyethylene glycol of the mass of the first liquid phase in step 3.

[0028] 250 mL of Na at pH 6.5 from step 4 2 HPO 4 and KH 2 PO 4 Phosphate buffer contains Na 2 HPO 4 6 grams, with KH 2 PO 4 4 grams. Dialyzed 3 times.

[0029] Onion enzyme freeze-dried powder 6.0g

Embodiment 3

[0031] According to the above process steps

[0032] In step 2, raw materials: 350 grams of scallion white, 500 ml of Na at pH 6.5 2 HPO 4 and KH 2 PO 4 Phosphate buffer contains Na 2 HPO 4 4.8 g, with KH 2 PO 4 2.8 g; 100 ml of glycerin, 1 g of disodium edetate, 0.0025 g of pyridoxal 5'-phosphate

[0033] 30% polyethylene glycol 8000 by mass of the first liquid phase in step 3

[0034] 500 mL of Na at pH 6.5 from step 4 2 HPO 4 and KH 2 PO 4 Phosphate buffer contains Na 2 HPO 4 4.8 g, with KH 2 PO 4 2.8 grams.

[0035] 5.3 g of onion enzyme freeze-dried powder was obtained.

[0036] Polyethylene glycol 8000 (PEG8000) can be preferably used in the above-mentioned embodiment.

[0037] Above-mentioned technique shows: adopt the glycerin of the phosphate buffer of pH6~pH6.8, 9%~12% of phosphate buffer volume, the disodium edetate of 1~4 g / thousand milliliters of phosphate buffer by adopting extracting solution After homogenizing with 5'-pyridoxal phosphate and / or...

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Abstract

The invention discloses a method for extracting onion enzyme from fistular onion stalks, which comprises the following steps: cooling fistular onion stalks at -4 DEG C to 4 DEG C, adding an extract and performing homogenization at -4 DEG C to 4 DEG C, filtering, separating at 0 DEG C to 5 DEG C to obtain a first solid phase and a first liquid phase; adding polyethylene glycol with a mass of 15%-30% of the mass of the first liquid phase into the first liquid phase at 0 DEG C to 5 DEG C, allowing the solution to stand, performing centrifugation at 0 DEG C to 5 DEG C to obtain a second solid phase and a second liquid phase; dissolving the second solid phase by a phosphate buffer with a pH of 6-6.8, dialyzing the solution, performing freeze drying to obtain the onion enzyme. A map obtainted by analyzing the extracted onion enzyme by SDS-PAGE shows that the onion enzyme is a mixture, wherein the relative molecular weights of components with high concentrations are mainly centralized between 40-55 kDa. The method for extracting onion enzyme from fistular onion stalks of the invention has simple and practical operations, and stable process quality.

Description

technical field [0001] The invention belongs to a method for extracting white scallion, in particular to a method for extracting allium enzyme from white scallion. Background technique [0002] Scallion white is the bulb of Allium fistulosum L.var.caespitosum Makino., a plant of Liliaceae. Pharmacological studies have shown that the chemical components contained in scallions are mainly organic sulfur-containing compounds and unsaturated fatty acids. Scallion white extract can significantly improve the frequency and degree of angina pectoris in patients with coronary heart disease and angina pectoris. It has a good effect of expanding coronary arteries and improving myocardial ischemia; lowering blood fat and cholesterol, resisting oxidative damage, protecting vascular endothelium, and resisting atherosclerosis. ; It has obvious anti-oxidative damage, inhibits the proliferation of vascular smooth muscle cells, protects endothelial cells, and reduces restenosis after balloon ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00
Inventor 张耕程璐胡松
Owner 张介眉
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