Method for screening protein aptamers by using micro-fluidic chip

Abstract

The invention discloses a method for screening protein aptamers by using a micro-fluidic chip. The method comprises steps that: a preset initial nucleic acid library is first incubated with a non-target protein, such that an optimized nucleic acid library is obtained; a target protein is incubated with microspheres, the target-protein-modified microspheres are positioned in a channel of a micro-fluidic chip; the optimized nucleic acid library is delivered into the channel of the micro-fluidic chip; the materials are processed through washing and eluting, such that an initial screened nucleic acid library is obtained; PCR amplification is carried out upon the initial screened nucleic acid library, the amplification products are separated through an affinity column, and the products are processed from denaturative melting and desalting, such that a primary nucleic acid library is obtained; the primary nucleic acid library is used for replacing the initial nucleic acid library, and the previous steps are repeated until a nucleic acid library containing target aptamers is obtained. The method provided by the invention has advantages of good versatility, short screening time, high screening efficiency, and good screening effect.

Description

technical field [0001] The invention relates to a method for screening nucleic acid aptamers, in particular to a method for screening target protein-specific nucleic acid aptamers using a specific device. Background technique [0002] Nucleic acid aptamer (aptamer), also known as nucleic acid aptamer, is screened by a new type of in vitro screening technology - systematic evolution of exponentially enriched ligands (SELEX), which is obtained from random single-stranded oligomeric The single-stranded oligonucleotides obtained from the nucleotide library that can specifically bind to the target substance can be DNA or RNA, and the length is generally 25-60 nucleotides. The emergence of nucleic acid aptamers has broken the traditional understanding that nucleic acids are only genetic information storage and transport carriers. Using the diversity of nucleic acid structures, nucleic acid aptamers can efficiently and specifically bind various target molecules. Nucleic acid aptam...

AI Technical Summary

Problems solved by technology

However, since the number of screening rounds in the conventional SELEX method is generally 8 to 20 rounds, and sometimes even 30 rounds are required to screen out an applicable nucleic acid aptamer, the number of nucleic acid aptamers that have been screened out is relatively small, only a few hundred kind

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