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Method for screening bacillus licheniformis capable of resisting diammonium phosphate

A technology of Bacillus licheniformis and diammonium hydrogen phosphate, which is applied in the field of microorganisms and can solve problems such as restricting the application of Bacillus licheniformis

Inactive Publication Date: 2014-03-05
WIN WIN GROUP
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  • Description
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Problems solved by technology

This limits the use of Bacillus licheniformis in fertilizers

Method used

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  • Method for screening bacillus licheniformis capable of resisting diammonium phosphate

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Embodiment Construction

[0012] The present invention will be described in further detail below through specific examples, so as to help to understand the content of the present invention.

[0013] The source of Bacillus licheniformis was purchased from the China General Microorganism Culture Collection (CGMCC) of the Institute of Microbiology, Chinese Academy of Sciences, with the number 1.0518.

[0014] Isolation basal medium used: nutrient agar medium

[0015] Peptone: 10 grams

[0016] Beef powder: 3.0 grams

[0017] Sodium chloride: 5.0 grams

[0018] Water: 1000ml

[0019] pH value: 7.3±0.1

[0020] Screening method:

[0021] (1) Preparation: Prepare the medium according to the standard of nutrient agar medium, together with the packaged petri dish, test tube medium, sterile water, glass scraper, pipette and other auxiliary equipment, after high temperature sterilization, it will be ready for use. Pour the sterilized nutrient agar medium onto a flat plate, place the test tube culture mediu...

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Abstract

The invention belongs to the microorganism field, and relates to a method for screening bacillus licheniformis capable of resisting diammonium phosphate, which comprises the following process: (1) screening; taking nutrient agar as a medium to perform a slant tube cultivation to bacillus licheniformis with the CGMCC preservation No. 1.0518, then carrying out a slant culture vessel marking cultivation to screen typical single colony of the bacillus licheniformis; moving the typical bacillus licheniformis to the inclined plane of the nutrient agar test tube for culturing; (2) acclimatizing: adding diammonium phosphate in the nutrient agar medium to prepare the diammonium phosphate medium with different concentration, gradually raising the diammonium phosphate concentration to perform a screening and acclimatizing cultivation for screening bacillus licheniformis capable of resisting the diammonium phosphate with high concentration, acquiring bacillus licheniformis capable of resisting the diammonium phosphate with mass concentration of 20%. The bacillus licheniformis can be used for fertilizer.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a method for screening and domesticating Bacillus licheniformis and the obtained Bacillus licheniformis, which are applied in fertilizers to improve the tolerance of Bacillus licheniformis to diammonium hydrogen phosphate. Background technique [0002] Bacillus licheniformis, Latin scientific name Bacillus licheniformis, is a kind of Bacillus. Bacillus licheniformis cells are rod-shaped and solitary in shape and arrangement, can produce anti-active substances, and have a unique mechanism of biological oxygen capture, which can inhibit the growth and reproduction of pathogenic bacteria. [0003] Bacillus licheniformis can inhibit the reproduction of pathogenic bacteria in the soil and the invasion of plant roots, reduce plant soil-borne diseases, and prevent the outbreak of various pests, so it is used in fertilizers. For example, CN101955903A discloses "a Bacillus licheniformis and i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/10
Inventor 冯作山尹川李宝成周正峰解丽娟
Owner WIN WIN GROUP
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