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Method of immobilizing phospholipase A1 by oxidation of sodium periodate for degumming of soybean crude oil

A technology of sodium periodate and phospholipase, applied in the direction of fat oil/fat refining, fat production, etc., can solve the problems of difficult repeated or continuous use, poor degumming effect of soybean oil, difficult separation and recovery, etc., to achieve the optimal response Range of conditions, enhanced activity and stability, low-cost effects

Inactive Publication Date: 2013-03-13
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that the temperature and pH of the existing enzyme degumming method are not easy to control, which leads to the poor degumming effect of soybean oil, the high cost of the existing enzyme degumming method, and the miscibility of natural enzymes in the reaction system. Not only is it difficult to separate and recover, but also the enzyme It is generally difficult to prepare, and the cost is high, and it is difficult to repeatedly or continuously use problems, and it is proposed to use sodium periodate oxidation method to immobilize phospholipase A 1 Applied in soybean crude oil degumming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Oxidative activation of sodium periodate: suspend a piece of cellulose filter paper membrane in 10ml of sodium periodate solution with a concentration of 0.01-0.3mol / L, shake it in a shaker at room temperature for 30-100min, take it out immediately Wash with deionized water until neutral, blot dry;

[0024] 2. Phospholipase A 1 Immobilization: take 1cm 2 The cellulose filter paper membrane is placed in a plate, and under the condition of 5-35°C, it is exchanged with 0.01-0.09g / mL enzyme solution and phosphate buffer solution with a pH of 5.8, and a volume concentration of 0.05-0.6% glutaraldehyde is added. After the cross-linking reaction, the cellulose filter paper membrane was suspended in 10 ml of sodium borohydride solution with a volume concentration of 1%, reacted at 4°C for 20-40 min, and then used 0.1 mol of HAI solution, 2 mol KCl solution and water were washed three times, dried and cut into pieces to obtain immobilized phospholipase A 1 ;

[0025] 3. S...

Embodiment 2

[0030] The difference between this embodiment and specific embodiment 1 is that in step 3, a citric acid solution with a mass concentration of 41%-44% is added to the treated soybean oil and stirred at a speed of 1100-1400 r / min for 9-16 minutes. Other steps are the same as in the first embodiment.

Embodiment 3

[0032] The difference between this embodiment and specific embodiment 1 is that in step 3, a citric acid solution with a mass concentration of 42-43% is added to the treated soybean oil and stirred at a speed of 1200-1300 r / min for 11-14 minutes. Other steps are the same as in the first embodiment.

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Abstract

Conventional enzymatic degumming methods have the following problems: degumming of soybean oil has a poor effect because temperature and pH values are hard to control, cost for the methods is high, natural enzyme mixed and dissolved in a reaction system is difficult to separate and recover, preparation of enzyme is generally difficult and cost a lot, it is difficult to repeatedly or continuously use the enzyme, etc; the invention aims to overcome the above-mentioned problems and provides a method of immobilizing phospholipase A1 by oxidation of sodium periodate for degumming of soybean crude oil. The method comprises the processes of oxidative activation of sodium periodate, immobilization of phospholipase A1 and degumming of soybean crude oil. According to the invention, phospholipase A1 immobilized by oxidation of sodium periodate is applied in degumming of soybean crude oil, which enables temperature and pH values to be controllable, thereby enlarging the optimum reaction condition scope of enzyme and allowing enzyme to maintain good activity in a wider range; the invention is applicable to large scale industrial operation.

Description

Technical field: [0001] The invention relates to a sodium periodate oxidation method immobilized phospholipase A 1 Method for degumming crude soybean oil. Background technique: [0002] Soybean oil contains about 3% phospholipids, which are directly related to the flavor and stability of oil products and the phenomenon of oil foaming and brain discharge during use. The phospholipids to be removed in the degumming process can be roughly divided into hydration Phospholipids (hy-dratable Phospholipids, HP) and nonhydratable phospholipids (Nonhydratable Phospholipids, NHP), both have certain differences in properties and removal processes. Hydrated phospholipids are easier to remove than non-hydrated phospholipids. The main existing soybean oil degumming method is enzymatic degumming. Enzymatic degumming is a high-tech in oil refining. This technology has great application value in improving the economic benefits and environmental performance of the oil industry. Enzyme degum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B3/00C11B3/04
Inventor 于殿宇马莺李琳刘晶李红玲周晓丹常云鹤孙博王玉李振岚齐颖于博涵
Owner HARBIN INST OF TECH