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Microbial cell immobilizing carrier and related application

A technology of microbial cells and immobilized carriers, applied to microorganisms, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve the problems of immobilization of new biomimetic materials, etc., It is suitable for large-scale popularization and application, with high immobilization efficiency and ingenious design.

Inactive Publication Date: 2012-03-14
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report on the use of this new type of bionic material for cell immobilization

Method used

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  • Microbial cell immobilizing carrier and related application
  • Microbial cell immobilizing carrier and related application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Taking Nano Iron Oxide as an Example to Immobilize Escherichia coli

[0018] 1. Dissolve 1g of dopamine hydrochloride in 500ml of pure water, add 1.0g of magnetic nano iron oxide, adjust the pH to 8.5 with 0.1mol / L NaOH, stir for 1 hour, separate with a magnet, wash the solid twice with pure water, The control group was magnetic nano-iron oxide without dopamine hydrochloride.

[0019] 2. Add water to Escherichia coli (E.coli) to make OD 600 For a solution of about 20, take 5ml of bacteria and add it to the above 5ml magnetic iron oxide material, shake at room temperature (25°C) for 30 minutes, separate the solid with a magnet, wash the solid twice with pure water, measure the OD value of the supernatant, and pass The indirect method obtained immobilized Escherichia coli (E. coli), the immobilization rate of iron oxide of dopamine hydrochloride was 96.4%, and the immobilization rate of the control group was 79.6%.

[0020] 3. Determination of cell immobilizat...

Embodiment 2

[0023] Example 2 Taking Nano Iron Oxide as an Example to Immobilize Yeast

[0024] 1. Dissolve 1g of dopamine hydrochloride in 500ml of pure water, add 0.5g of magnetic nano iron oxide, adjust the pH to 8.5 with 0.1mol / L NaOH, stir for 1 hour, separate with a magnet, wash the solid twice with pure water, and compare The group is magnetic nano-iron oxide without dopamine hydrochloride.

[0025] 2. Add water to the yeast to make OD 600 For a solution of about 20, take 5ml of bacteria and add it to the above 5ml magnetic iron oxide material, shake at room temperature (25°C) for 30 minutes, separate the solid with a magnet, wash it twice with pure water, measure the OD value of the supernatant, and calculate The obtained immobilized yeast had an immobilization rate of iron oxide containing dopamine hydrochloride of 95.6%, and the immobilization rate of the control group was 80.3%.

[0026] 3. Activity evaluation: transforming L-phenylalanine by yeast to generate 2-phenylethanol....

Embodiment 3

[0027] Example 3 Taking Nano Iron Oxide as an Example to Immobilize Gluconobacter oxidans

[0028] 1. Dissolve 1g of dopamine hydrochloride in 500ml of pure water, add 0.5g of magnetic nano iron oxide, adjust the pH to 8.5 with 0.1mol / L NaOH, stir for 1 hour, separate with a magnet, wash the solid twice with pure water, The control group was magnetic nano-iron oxide without dopamine hydrochloride.

[0029] 2. Add water to the wet cell G of Gluconobacter oxydans to make OD 600 For the solution of 3, take 5ml of the bacterial solution and add it to the above-mentioned 5ml magnetic iron oxide material, shake at room temperature (25° C.) for 30 minutes, separate the solid with a magnet, and wash it twice with pure water to obtain immobilized Gluconobacter oxidans. The OD value of the supernatant was measured, and the immobilization rate was calculated to be 98.9%, and the immobilization rate of the control group was 84.8%.

[0030] 3. Activity evaluation: 2-hydroxyacetone was ge...

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Abstract

The invention relates to a microbial cell immobilizing carrier. The carrier is prepared by modifying an inorganic carrier or organic carrier with a compound containing a catechol structure, preferably, the compound containing the catechol structure is DOPA, dopamine, 3,4-dihydroxybenzylamine, 3,4-dihydroxybenzadehyde, 3,4-dihydroxyphenylacetaldehyde, 3,4-dihydroxybenzoic acid or 3,4-dihydroxyphenylacetic acid; the inorganic carrier is diatomite, silica gel powder, lake corallite, molecular sieve, kaoline, metal oxide, metal oxide nano-particles, microporous ceramics or microporous glass; and the organic carrier is high-molecular-weight polymer or polysaccharide. The invention also provides application of the microbial cell immobilizing carrier in microbial cell immobilization. The microbial cell immobilizing carrier provided by the invention has smart design, so that microbial cells can be immobilized on the organic or inorganic carrier simply and conveniently at high immobilization efficiency, and the cell activity is not influenced. The microbial cell immobilizing carrier is suitable for large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of cell immobilization, in particular to the technical field of microbial cell immobilization, and more specifically to a microbial cell immobilization carrier and related applications. Background technique [0002] Immobilization of cells plays an important role in industrial applications of cell catalysis. Currently, there are mainly two methods of cell immobilization, physical and chemical (Food Microbiology (2004), 21: 377-397). Physical methods mainly use organic and inorganic porous materials to adsorb or embed macromolecules to immobilize cells. Adsorption and immobilization of cells is to use the electrostatic attraction between the porous material and the cell surface to fix the cells on the surface of the porous material. Although it has the least effect on the activity of the cells, the immobilization efficiency is not high, and the weak force between the cells and the carrier As a resul...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/12C12N11/10C12N11/08C12R1/01C12R1/845C12R1/785C12R1/80C12R1/66C12R1/885C12R1/745C12R1/84C12R1/19C12R1/02C12R1/32C12R1/225
Inventor 任宇红王存勋周勖鲁慧敏倪克奉魏东芝
Owner EAST CHINA UNIV OF SCI & TECH
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