Method for inhibiting azotobacter in intestinal canal of termite by utilizing metabolic products of tree endophyte
A metabolite inhibition, endophyte technology, applied in microorganism-based methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve problems such as large toxic side effects, environmental pollution, human harm, etc. Long, small environmental impact, good killing effect
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specific Embodiment approach 1
[0011] Specific embodiment one: the present embodiment utilizes tree endophyte metabolites to suppress the method for termite intestinal nitrogen-fixing bacteria, proceed according to the following steps:
[0012] 1. Pick a ring of tree endophytes, inoculate it into beef extract peptone medium, and cultivate it at 37°C and 180r / min for 2-3 days to obtain a culture solution of tree endophytes; Centrifuge the culture solution at 5000r / min for 10-15min, take the supernatant, and concentrate it to one-twentieth of its volume under the conditions of aseptic, 35°C constant temperature water bath and 150r / min to obtain the endophyte fermentation broth 2. Prepare plain agar plates: add 20 g of agar to 1000 mL of water, sterilize to obtain plain agar, then pour 10 to 15 mL of plain agar into each plate to obtain plain agar plates, and stand the Oxford cup on the plain agar plates; 3. Preparation of bacterial suspension: pick a ring of termite intestinal nitrogen-fixing bacteria, inocul...
specific Embodiment approach 2
[0018] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the nitrogen-free slant culture medium described in step three is made of 15g sucrose, 0.8g KH 2 PO 4 , 0.2g MgSO 4 , 0.2g NaCl, 1g CaCO 3 , 20g agar, 1mL mass concentration of 10% Na 2 MO 4 solution, 1 mL of H 3 BO 3 solution, 1 mL of MnSO 4 solution, 1 mL of Fe 2 SO 4 solution and 1000mL H 2 O composition, adjust the pH to 6.5-7.0, and sterilize at 121°C for 30 minutes. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0019] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the nitrogen-free liquid culture medium described in step four is made of 15g sucrose, 0.8g KH 2 PO 4 , 0.2g MgSO 4 , 0.2g NaCl, 1g CaCO 3 , 1mL of Na with a mass concentration of 10% 2 MO 4 solution, 1 mL of H 3 BO 3 solution, 1 mL of MnSO 4 solution, 1 mL of Fe 2 SO 4 solution and 1000mL H 2 O composition, adjust the pH to 6.5-7.0, and sterilize at 121°C for 30 minutes. Others are the same as in the first or second embodiment.
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