Method for preparing Cerasus humilis kernel extract

A technology of eucalyptus and extract, applied in the field of preparation of eucalyptus extract, can solve problems such as denaturation of eucalyptus protein, affecting the in-depth development and utilization of eucalyptus protein, etc.

Inactive Publication Date: 2013-05-08
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, high temperature treatment led to severe denaturation of eulisin, which affected the in-depth development and utilization of eulerenin

Method used

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  • Method for preparing Cerasus humilis kernel extract
  • Method for preparing Cerasus humilis kernel extract
  • Method for preparing Cerasus humilis kernel extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Drying 100 g of Prunus oleifera by sun-drying so that the water activity of the Prunus oleifera is 0.70 to obtain dried Prunus prune;

[0029] According to the Soxhlet extraction method, the Prunus oleifera oil as raw material is determined, the amygdalin in Prunus punctifera as a raw material is determined by high performance liquid chromatography, and the amygdalin in Prunus punctifera as a raw material is determined according to the Kjeldahl method. As can be seen, the mass percentages of oil, amygdalin and protein are respectively 39.8%, 3.45% and 28.6%;

[0030] 2) Under the condition of keeping the water activity of the dried Echinacea obtained in step 1) at 0.70, crush the dried Euliscus to a size of 10 mesh to obtain 97.1 g of crushed Eulison;

[0031] 3) Under the condition that the water activity of the pulverized Prunus obtained in step 2) was kept at 0.70, the Prunus oil in the pulverized Prunus was extracted with an organic solvent at 50° C. to obtain 38...

Embodiment 2

[0042] 1) Dehydrating 100 g of Prunus oleifera with 20 mL of ethanol as a dehydrating agent, so that the water activity of Prunus oleifera is 0.53 to obtain dried Prunus prune;

[0043] According to the Soxhlet extraction method, the Prunus oleifera oil as raw material is determined, the amygdalin in Prunus punctifera as a raw material is determined by high performance liquid chromatography, and the amygdalin in Prunus punctifera as a raw material is determined according to the Kjeldahl method. As can be seen, the mass percentages of oil, amygdalin and protein are respectively 39.8%, 3.45% and 28.6%;

[0044] 2) Under the condition that the water activity of the dried Euliscus obtained in step 1) was kept at 0.53, the dried Euliscus was crushed to 120 mesh to obtain 95.26 g of the crushed Euliscus;

[0045] 3) Under the condition that the water activity of the pulverized Eurydene obtained in step 2) is kept at 0.53, use supercritical CO at 42°C to 2 The method of fluid extrac...

Embodiment 3

[0056] 1) Dry 100 g of Prunus oleifera by freeze-drying method, so that the water activity of Prunus oleifera is 0.40, and obtain dried Prunus mellifera; The vacuum freeze-drying operation is carried out at a temperature of 20-40Pa, and the freeze-drying is completed when the temperature of the material is close to room temperature.

[0057]According to the Soxhlet extraction method, the Prunus oleifera oil as raw material is determined, the amygdalin in Prunus punctifera as a raw material is determined by high performance liquid chromatography, and the amygdalin in Prunus punctifera as a raw material is determined according to the Kjeldahl method. As can be seen, the mass percentages of oil, amygdalin and protein are respectively 39.8%, 3.45% and 28.6%;

[0058] 2) Under the condition of keeping the water activity of the dried Euliscus obtained in step 1) at 0.40, crush the dried Euliscus to 60 meshes to obtain 94.68 g of the crushed Euliscus;

[0059] 3) Under the condition...

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Abstract

The invention discloses a method for preparing a Cerasus humilis kernel extract, comprising the following steps: 1) drying Cerasus humilis kernels to let the moisture activity of the Cerasus humilis kernels be 0.10-0.70; 2) crushing until the particle size is 10-120 meshes; 3) under the condition of keeping the moisture activity the same, extracting Cerasus humilis kernel oil; and 4) carrying outseparation and extraction on the Cerasus humilis kernel meal with an ethanol aqueous solution having a percentage concentration of 50-95 % to obtain amygdalin and protein from Cerasus humilis kernel.According to the invention, when crushing Cerasus humilis kernels and extracting Cerasus humilis kernel oil, the activity of amygdalase is reduced by controlling the moisture activity; when the separation and extraction of amygdalin and protein from Cerasus humilis kernel, the activity of amygdalase is reduced and excess denature of the protein from Cerasus humilis kernel is prevented by controlling the concentration and operation temperature of ethanol, thus the full utilization of the Cerasus humilis kernel oil, the protein from Cerasus humilis kernel and amygdalin is realized, and the invention lays a foundation for deep processing and comprehensive utilization of Cerasus humilis kernels.

Description

technical field [0001] The invention relates to a method for preparing the extract of Prunus japonicus. Background technique [0002] Prunus humilis Bunge is a small deciduous shrub of the genus Cherry in the family Rosaceae. It is a wild fruit tree that is resistant to cold, drought, barrenness, salt and alkali, and has strong adaptability. It is a unique wild tree species for both fruit and medicine in my country. Ou Li is mainly distributed in Shanxi, Liaoning, Hebei, Shandong, Inner Mongolia, Northeast China, Shaanxi and other places. In the Sanbei area, within the range of 500-1500m above sea level, 500 plants are planted per 667m2. Generally, it enters the full fruit period in the third year, and the yield per mu can reach 500-1000kg. The fruit production cycle is short and the yield is high. [0003] The main components of prunes include amygdalin, prunes oil and prunes protein. Prunus amygdalin contains 2%-5% amygdalin, amygdalin has antitussive and asthma, anti-tu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C07H1/08C07K1/14C11B1/04C11B1/00C11B1/10C11B1/06
CPCY02P20/544Y02P20/54
Inventor 田金强兰彦平周连第于艳静周家华常虹
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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