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Device for preparing and collecting cell microspheres on basis of type I collagen gel particles

A technology of collagen gel particles and cells, which is applied in the field of biomedical engineering, can solve problems such as unsatisfactory, and achieve the effects of overcoming differences, increasing content, and changing the passage mode

Active Publication Date: 2013-05-22
HANGZHOU BAIQIAO MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The collection and inoculation of seed cells are still done manually by centrifugation, which cannot meet the needs of tissue engineering to construct complex tissues

Method used

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  • Device for preparing and collecting cell microspheres on basis of type I collagen gel particles

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Experimental program
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Embodiment Construction

[0017] The present invention will be further described below in conjunction with accompanying drawing.

[0018] Such as figure 1 As shown, the device for preparing and collecting cell microspheres based on type I collagen gel particles includes three parts: a cell microsphere expansion preparation and collection device, a control unit and a measurement unit.

[0019] Cell microsphere expansion preparation and collection device: It consists of three parts: cell microsphere expansion culture room, culture solution fluidized bed lifting layer flow circulation mechanism, and cell microsphere automatic collection mechanism.

[0020] The flow circulation mechanism of the fluidized bed lifting layer of the culture medium can form a stable lifting layer of the laminar flow of the fluidized bed of the culture medium, so as to ensure that the cells and type I collagen gel particles can be suspended and move stably on the lifting layer, providing the cells in the I The adhesion on type ...

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Abstract

The invention relates to a device for preparing and collecting cell microspheres on the basis of type I collagen gel particles. In the prior art, the usual seed cell amplification method still adopts the manual culture. A culture fluid fluidized bed support layer flow circulation mechanism in the device forms a stable culture fluid fluidized bed layer flow support layer, cells and type I collagengel particles can be ensured to be stably suspended on the support layer to move, a cell microsphere amplification culture chamber is positioned above the culture fluid fluidized bed support layer flow circulation mechanism and is used for cell microsphere preparation and cell amplification, and a cell microsphere automatic collecting mechanism is used for collecting cell microspheres after the cell microsphere preparation and the culture are completed. The device has the advantages that the culture fluid flowing is adopted to form the fluidized bed support layer for cell microsphere suspension culture amplification, the cell epimatrix content is increased, moving rotational flows are adopted for collecting the cell microspheres, and the defect caused by the adoption of the centrifugal method is overcome.

Description

technical field [0001] The invention belongs to the field of biomedical engineering and relates to a device for in vitro amplification preparation and automatic collection of cell microspheres. In particular, it relates to a method and a device for preparing cell microspheres by composite expansion culture of cells and type I collagen gel particles, and then coating the cell microspheres with type I collagen gel to form active silk threads. It is suitable for the large-scale expansion of cells in vitro in the field of biomedical engineering and the preparation of cell microsphere filaments with cell activity. It is applied to the precise inoculation of complex tissues and organs in tissue engineering and cell therapy in regenerative medicine. Background technique [0002] In the clinical application of tissue engineering and regenerative medicine, a large number of seed cells are needed, and these seed cells need to be inoculated on specific biomaterials for shaping. Since t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/36
CPCC12M25/20C12M33/00C12M41/36
Inventor 李宏
Owner HANGZHOU BAIQIAO MEDICAL TECH CO LTD