Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Functional molecular marker I for identifying muskmelon fusarium wilt resistance and application thereof

A melon wilt, molecular marker technology, applied in the direction of microbial determination/inspection, DNA/RNA fragment, recombinant DNA technology, etc., can solve problems such as affecting accuracy

Inactive Publication Date: 2012-04-04
ZHEJIANG UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a certain genetic distance between the molecular markers used in the current disease resistance breeding process and the target gene, which is prone to recombination and exchange and separation, thus affecting the accuracy of selection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Functional molecular marker I for identifying muskmelon fusarium wilt resistance and application thereof
  • Functional molecular marker I for identifying muskmelon fusarium wilt resistance and application thereof
  • Functional molecular marker I for identifying muskmelon fusarium wilt resistance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, main steps and contents of the present invention are: design primers according to muskmelon wilt resistance gene Fom-2 in the database, use muskmelon disease-resistant and susceptible germplasm and their F 1 , F 2 Genomic DNA of the first generation is used as a template, and the target fragment is amplified by chain polymerase reaction (PCR), sequenced, and then the sequence is analyzed by applying bioinformatics. Two methods of CAPS and AS-PCR were used to develop FMs related to Fusarium wilt resistance, and in F 1 , F 2 Generations have proven its validity and reliability. Using the above two marker methods, the genomic DNA of different melon germplasms used in production was extracted, and the genotypes of resistance to Fusarium wilt were identified.

[0038] Specific steps are as follows:

[0039] (1) Find the sequence information of the muskmelon wilt resistance gene Fom-2 in the NCBI database (http: / / www.ncbi.nlm.nih.gov / ), and the accession num...

Embodiment 2

[0147] A total of 34 varieties of thin-skinned and thick-skinned melons with large cultivation areas in Zhejiang and local characteristic varieties were selected (Table 3). The resistance to Fusarium wilt was screened by AS-PCR and CAPS.

[0148] In the CAPS method, we use specific amplification primers: forward primer CAPS2-F: 5'-GGAAGTGAGG-TGTTGAATT-3' and reverse primer CAPS2-R: 5'-TACACATTGGTCCGTTAGAC-3'), to test the species genome DNA is used as a template for specific PCR (reaction system and PCR reaction conditions are the same as above). After purification, the product was digested with EcoR I restriction endonuclease, HAD-GT12 TM The automatic capillary nucleic acid analysis system performs electrophoresis analysis, and records and analyzes the results after data correction.

[0149] In the AS-PCR method, we use specific amplification primers: forward primer 2-3F1: 5'-GTGTAACACAAA-TTCCTCAACA-3' and reverse primer 2-3R1: 5'-GTGTAACACAAATTCCTCATCA-3'), to be tested ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a functional molecular marker I for identifying muskmelon fusarium wilt resistance. A sequence of a primer used by the molecular marker is CAPS1, namely a forward primer is 5'-TGCTCCTTTGGCTTCCTGT-3', and a reverse primer is 5'-GAGTCTATTGTTTCGCTTCA-3'. The molecular marker I is used for the molecular auxiliary breeding of the muskmelon fusarium wilt or is used for identifying resistance genes of the muskmelon fusarium wilt.

Description

[0001] The present invention is a divisional application of "Functional Molecular Markers for Identification of Melon Fusarium Wilt Resistance and Its Application" with the filing date of 2010.04.15 and the application number of 201010147650.0. technical field [0002] The invention belongs to the technical field of molecular marker breeding for vegetable disease resistance, and in particular relates to a functional molecular marker of the muskmelon fusarium wilt resistance gene Fom-2 and its application, thereby providing a new type of molecular marker for marker-assisted selection of muskmelon wilt resistance . Background technique [0003] Melon (Cocumis melo L.), as an important horticultural crop in Cucurbitaceae, has high commercial value and economic value, and is one of the important economic crops in my country. In recent years, with the commercialization of melon germplasm resources and the continuous expansion of planting area, the occurrence of diseases and insec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 杨景华张明方王士伟
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com