Method for extracting and purifying sequoyitol

A technology for taxol and yew, which is applied in the field of extracting and purifying taxol, can solve problems such as unfavorable environmental protection, high production cost, difficult application and the like, and achieves the effects of protecting the environment, reducing production costs, and simplifying process steps

Active Publication Date: 2012-04-11
福建紫杉园生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing method for extracting and purifying paclitaxel from Taxus chinensis, the disadvantages are that the process steps are relatively complicated, it is relatively difficult to apply in industry, and a large amount of chemical solvents need to be used in the production process, and its production cost is relatively high. High and not conducive to environmental protection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Grind the yew raw material until the particle size is less than 0.85 mm, put the crushed yew raw material in an extraction container, add 30% (by weight) methanol solution for extraction, and leaching the yew raw material with each added The weight ratio of the methanol solution is 1:3; the leaching is repeated three times, and then the extracts are combined and concentrated in a vacuum at a temperature lower than 80° C. to recover the methanol to obtain the concentrated extract of Taxus chinensis. The concentrated yew extract is extracted with chloroform, the volume ratio of the concentrated yew extract to chloroform is 1:1, and paclitaxel liquid and raffinate are separated after extraction. Add the flocculant polyacrylamide to the raffinate, the volume ratio of the raffinate to the polyacrylamide is 1:0.01, then let it settle for 6 hours, centrifuge to remove impurities, and separate to obtain the paclitaxel liquid, the paclitaxel medicine The solution was concentrate...

Embodiment 2

[0023] Crush the yew raw material until the particle size is less than 0.85 mm, put the crushed yew raw material in an extraction container, add ethanol for extraction, the weight ratio of the yew raw material to the ethanol added each time is 1:5; repeat After leaching for 5 times, the extracts were combined and concentrated in a vacuum at a temperature lower than 80° C. to recover ethanol to obtain a concentrated extract of Taxus chinensis. The concentrated extract of yew is extracted with ethyl acetate, the volume ratio of the concentrated extract of yew to ethyl acetate is 1:3, and paclitaxel liquid and raffinate are separated after the extraction is completed. Add flocculant polyacrylamide to the raffinate, the volume ratio of raffinate to polyacrylamide is 1:0.05, let it settle for 10 hours, centrifuge to remove impurities, and separate to obtain paclitaxel liquid, paclitaxel liquid The crude product of Seclitaxel was obtained after concentration and drying. Dissolve th...

Embodiment 3

[0025] Crush the yew raw material until the particle size is less than 0.85 mm, put the crushed yew raw material in an extraction container, add acetone for extraction, the weight ratio of the yew raw material to the acetone added each time is 1:4; repeat After leaching for 4 times, the extracts were combined and concentrated in a vacuum at a temperature lower than 80° C., acetone was recovered, and the concentrated extract of Taxus chinensis was obtained. The concentrated yew extract is extracted with chloroform, the volume ratio of the concentrated yew extract to chloroform is 1:2, and paclitaxel liquid and raffinate are separated after extraction. Add flocculant polyacrylamide to the raffinate, the volume ratio of raffinate to polyacrylamide is 1:0.03, let it settle for 8 hours, centrifuge to remove impurities, and separate to obtain paclitaxel liquid, paclitaxel liquid The crude product of Seclitaxel was obtained after concentration and drying. Dissolve the crude product ...

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PUM

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Abstract

The invention relates to a method for extracting and purifying sequoyitol. The method comprises the following steps: grinding a Taxus chinensis raw material, preparing a Taxus chinensis concentrated extract, separating out a taxol medical solution, adding a flocculant to prepare crude sequoyitol, preparing sequoyitol crystals, preparing high-purity sequoyitol, and the like. In the method, after the taxol medical solution is separated out from the Taxus chinensis raw material, the flocculant is added in the faffinate and the solution is treated to directly prepare crude sequoyitol, thus the Taxus chinensis raw material can be comprehensively utilized, the processing steps for preparing crude sequoyitol can be simplified and the production cost of the sequoyitol extraction process can be reduced; and the method is more suitable for industrial production and is beneficial to environmental conservation.

Description

technical field [0001] The invention relates to the technical field of extracting and purifying paclitaxel, in particular to a method for extracting and purifying paclitaxel from yew plant raw materials. Background technique [0002] Taxus chinensis has excellent medicinal value, which is mainly reflected in the extraction of anti-cancer substance - paclitaxel. Modern pharmaceutical research has proved that another ingredient in yew—taxol has obvious hypoglycemic effect and low toxicity, which can be used in the treatment of diabetes. [0003] At present, there is a method for extracting and purifying paclitaxel from Taxus chinensis, for example, in a Chinese patent whose authorized announcement number is CN101244987B and the title of the invention is "a method for extracting and purifying paclitaxel by solvent reflux", it discloses A method for extracting and purifying paclitaxel from yew raw materials, comprising pulverizing yew raw materials, preparing yew concentrated e...

Claims

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Application Information

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IPC IPC(8): C07C43/196C07C41/34
Inventor 李长明彭家文
Owner 福建紫杉园生物有限公司
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