Salt-tolerant marine mangrove endophytic fungi with neurodegenerative disease control activity
A neurodegenerative, endophytic fungal technology, applied in nervous system diseases, fungi, microorganisms, etc., can solve the problems of irreversible disease, adverse reactions of acetylcholine, etc., and achieve strong neurodegenerative disease prevention and treatment activity, strong anti-AD activity Effect
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Embodiment 1
[0027] Example 1 Isolation of marine mangrove salt-tolerant endophytic fungi from mangrove bark
[0028]The bark samples of the mangrove plant Punggia chinensis collected near the water surface of the mangroves in the Shenzhen sea area are screened and cultured in the laboratory according to the following conditions, and the marine mangrove salt-tolerant endophytic fungus SN3 described in this patent can be obtained -2.
[0029] (1) Culture medium and preparation:
[0030] (1) PDA solid medium: Wash and peel 200g of potatoes, add 800ml of water, boil for 30min, and filter through six layers of gauze; add 15g of agar to the filtrate, add 20g of glucose, the final concentration of penicillin is 80u / ml, and the final concentration of streptomycin is 36u / ml. 0.1MPa, sterilized at 121°C for 30min, glucose, penicillin and streptomycin were sterilized by filtration.
[0031] Penicillin specification: 1600u / mg, packed in 5g. Preparation method: Weigh 500mg and dissolve it in 10ml...
Embodiment 2
[0048] Example 2 Salt-tolerant endophytic fungi of marine mangroves Salt-tolerant, halophilic and seepage-resistant analysis
[0049] Inoculate the isolated marine mangrove salt-tolerant endophytic fungi into PDA solid high-salt medium containing NaCl concentrations of 1M, 2M, and 3M, culture them in a dark room at 25°C, and record their growth status after 7 days, as shown in Table 2 .
[0050] Table 2 Salinity identification results of marine mangrove salt-tolerant endophytic fungi to NaCl
[0051]
[0052] Note: A "+" indicates that the colony covers 1 / 4 of the plate, and a "-" indicates that the colony does not grow.
[0053] The purified marine mangrove salt-tolerant endophytic fungi were inoculated on PDA solid medium plates containing 1M NaCl / salt-free, cultured in a dark room at 25°C, and the growth conditions were recorded to detect whether there were halophilic bacteria. The results are shown in Table 3. It can be seen that SN3-2 is a non-halophilic bacteria.
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Embodiment 3
[0062] Example 3 Isolation of genomic DNA from marine mangrove salt-tolerant endophytic fungi
[0063] The results of agarose gel electrophoresis of genomic DNA isolated from salt-tolerant endophytic fungi of marine mangroves are shown in figure 2 .
[0064] The 18SrDNA was isolated from the genomic DNA of the salt-tolerant endophytic fungus of the marine mangrove, and its base sequence is shown in SEQ ID NO:1.
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