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Tool and box for quickly detecting early-stage puerperal convulsion by taking Adipsin as detection index and manufacturing method thereof

A preeclampsia and detection tool technology, applied in measurement devices, instruments, disease diagnosis and other directions, can solve the problems of undiscovered preeclampsia detection tools and detection boxes, time-consuming, cumbersome sample collection methods, etc., to facilitate industrial production, Simple operation and simple structure effect

Active Publication Date: 2014-01-29
ORIGISSAY BIOLOGICS TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of collecting samples by this method is cumbersome and time-consuming
There are no reports of preeclampsia detection tools and detection kits that use the Adipsin content in urine samples as the detection index

Method used

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  • Tool and box for quickly detecting early-stage puerperal convulsion by taking Adipsin as detection index and manufacturing method thereof
  • Tool and box for quickly detecting early-stage puerperal convulsion by taking Adipsin as detection index and manufacturing method thereof
  • Tool and box for quickly detecting early-stage puerperal convulsion by taking Adipsin as detection index and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] In this embodiment, the rapid detection tool for preeclampsia using Adipsin as a detection index such as figure 1 , figure 2 As shown, its shape is a rectangular strip, and its structure includes a bottom plate 1, an absorbent pad 2, a nitrocellulose membrane 5, a gold standard pad 6, and a sample pad 7, and the absorbent pad, nitrocellulose membrane, gold standard pad, and sample pad Paste on the bottom plate successively from top to bottom, and the gold standard pad is partially covered by the sample pad. Bottom plate 1 is made of medical plastic high-whiteness PVC sheet, coated with pressure-sensitive adhesive, which is inert to biologically active molecules; water-absorbing pad 2 is made of water-absorbing paper with good water absorption performance, model H5076; The detection line 4 formed by the rabbit anti-human Adipsin polyclonal antibody coating and the control line 3 formed by the goat anti-mouse IgG polyclonal antibody coating, the detection line 4 is loca...

Embodiment 2

[0063] In this embodiment, the rapid detection tool for preeclampsia using Adipsin as a detection index such as figure 1 , figure 2 shown. The difference from Example 1 is: the detection line 4 provided on the nitrocellulose membrane 5 is formed by coating with a goat anti-human Adipsin polyclonal antibody, and the width of the detection line 4 and the control line 3 is 0.5mm (or 0.7mm or 1mm), the distance between them is 5mm (or 7mm or 9mm); the gold standard pad 6 is made of polyester fiber membrane and coated with the gold mouse anti-human Adipsin monoclonal antibody conjugate; the sample pad is made of polyester fiber Pads made of membranes.

[0064] In the present embodiment, the preparation method of the rapid detection tool for preeclampsia using Adipsin as the detection index is as follows:

[0065] (1) Coating of detection line and control line

[0066] ① Use carbonate buffer solution with pH=9 and concentration of 0.05mol / L as solvent and goat anti-human Adipsin ...

Embodiment 3

[0075] In this embodiment, the rapid detection tool for preeclampsia using Adipsin as a detection index such as figure 1 , figure 2 shown. The difference from Example 1 is that the detection line 4 provided on the nitrocellulose membrane 5 is formed by coating with a mouse anti-human Adipsin monoclonal antibody, and the widths of the detection line 4 and the control line 3 are both 0.5mm or 0.8mm. The distance between them is 6mm or 10mm; the gold standard pad 6 is made of non-woven fabric and coated with the golden mouse anti-human Adipsin monoclonal antibody conjugate; the sample pad is a pad body made of non-woven fabric.

[0076] In the present embodiment, the preparation method of the rapid detection tool for preeclampsia using Adipsin as the detection index is as follows:

[0077] (1) Coating of detection line and control line

[0078] ①Using tris buffer solution with pH=7.4 and concentration of 0.05mol / L as solvent and mouse anti-human Adipsin monoclonal antibody as...

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Abstract

A means for rapidly detecting Adipsin for diagnosing preeclampsia comprises a water-adsorbing pad(2), a nitrocellulose membrane(5), a gold labeling pad (6) and a sampling pad (7), all of which are successively joined from the top down and fixed in the base plate(1). The gold-labeling pad (6) is partly overlapped by the sampling pad (7). A detecting line (4) coated by rabbit anti-human Adipsin polyclonal antibody, or goat anti-human Adipsin polyclonal antibody or mouse anti-human Adipsin polyclonal antibody and a controlling line (3) coated by goat anti-mouse IgG polyclonal antibody are provided on the nitrocellulose membrane (5). The detecting line (4) is located downstream and spaced from the controlling line (3).The gold-labeling pad (6) is made of water adsorbing material, and coated by gold labeled mouse anti-human Adispin monoclonal antibody. A tesk kit for rapidly detecting Adipsin for diagnosing preeclampsia comprises an outer house (8) and the said means therein. The method for preparing the said detecting means comprise: 1) coating of the detecting line (4) and the controlling line (3); 2) preparing the gold labeling pad (6); 3)combination.

Description

technical field [0001] The invention belongs to medical detection tools, in particular to a detection tool for detecting preeclampsia (PE for short) in pregnant women. Background technique [0002] Preeclampsia (PE for short) is an obstetric complication that occurs after 20 weeks of pregnancy and is one of the leading causes of maternal and perinatal death. The incidence rate of preeclampsia among pregnant women worldwide is 2-8%, and the incidence rate among pregnant women in my country can reach 10%. Patients with severe preeclampsia may have serious clinical manifestations such as hypertension, edema, and severe proteinuria, and are also prone to adverse outcomes such as pulmonary edema, renal failure, and HELLP syndrome, and suffer from long-term cardiovascular disease, kidney disease, and metabolic disease. The risk of disease is also significantly increased. For the fetus, the smaller the gestational age, the lower the survival rate. The mortality rate of premature ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/544G01N33/577G01N33/531G01N33/532
CPCG01N33/689G01N33/558G01N2333/96433G01N2800/368G01N33/54388
Inventor 胡怀忠
Owner ORIGISSAY BIOLOGICS TECH
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