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Fenugreek flavonoids extract preparation method and blood sugar level decreasing application thereof

A technology of fenugreek and extract, which is applied in the field of preparation of flavonoid extract, can solve the problems that have not been widely used, and achieve the effects of improving glucose tolerance, simplifying operation steps, and reducing industrial consumption

Inactive Publication Date: 2012-05-09
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Boiled and supercritical CO 2 Fluid extraction methods have many limitations and are not widely used

Method used

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  • Fenugreek flavonoids extract preparation method and blood sugar level decreasing application thereof
  • Fenugreek flavonoids extract preparation method and blood sugar level decreasing application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 The preparation method of fenugreek flavonoids extract comprises the following steps:

[0059] (1) Drying and crushing fenugreek seeds with a particle size of 60 mesh with a mass concentration of 70% ethanol solution at an extraction temperature of 60°C, an ultrasonic power of 600W, and a solid-liquid ratio of 1:20 (g / mL), Ultrasonic extraction was carried out under the condition that the extraction time was 2 hours to obtain an extract.

[0060] (2) After the extract is concentrated in vacuo at 50°C, the protein is removed by ethanol precipitation with a mass concentration of 90%, and repeated three times to obtain a concentrated solution; the concentrated solution is sequentially passed through ethanol with a mass concentration of 95% at a flow rate of 2BV / h , pure water, hydrochloric acid with a mass concentration of 5%, pure water, sodium hydroxide with a mass concentration of 2%, and a medium-polarity macroporous adsorption resin treated with pure wate...

Embodiment 2

[0065] The preparation method of embodiment 2 fenugreek flavonoids extract comprises the following steps:

[0066] (1) The fenugreek seeds that have been dried and pulverized into a particle size of 20 mesh are 10% ethanol solution at an extraction temperature of 20°C, an ultrasonic power of 100W, and a solid-liquid ratio of 1:5 (g / mL), Ultrasonic extraction was carried out under the condition that the extraction time was 0.5 h to obtain an extract.

[0067] (2) After the extract was concentrated in vacuo at 55°C, the protein was removed by ethanol precipitation with a mass concentration of 70%, and repeated three times to obtain a concentrated solution; the concentrated solution was sequentially passed through ethanol with a mass concentration of 95% at a flow rate of 2BV / h , pure water, hydrochloric acid with a mass concentration of 5%, pure water, sodium hydroxide with a mass concentration of 2%, and a medium-polarity macroporous adsorption resin treated with pure water.

...

Embodiment 3

[0072] The preparation method of embodiment 3 fenugreek flavonoids extract comprises the following steps:

[0073] (1) Drying and crushing fenugreek seeds with a particle size of 40 meshes with a mass concentration of 90% ethanol solution at an extraction temperature of 40°C, an ultrasonic power of 350W, and a solid-liquid ratio of 1:15 (g / mL), Ultrasonic extraction was performed under the condition that the extraction time was 1 h to obtain an extract.

[0074] (2) After the extract is vacuum-concentrated at 60° C., the protein is removed by ethanol precipitation with a mass concentration of 85%, and repeated three times to obtain a concentrated solution; the concentrated solution is sequentially passed through ethanol with a mass concentration of 95% at a flow rate of 2BV / h , pure water, hydrochloric acid with a mass concentration of 5%, pure water, sodium hydroxide with a mass concentration of 2%, and a medium-polarity macroporous adsorption resin treated with pure water. ...

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Abstract

The invention relates to a fenugreek flavonoids extract preparation method, which comprises the following steps: (1) dried and crushed fenugreek seeds are ultrasonically extracted through an ethanol solution, so the extracting solution is obtained; (2) the concentrated extracting solution is alcoholically precipitated through ethanol and is filtered, so the concentrated solution is obtained; the concentrated solution sequentially passes through the ethanol, purified water, hydrochloric acid, purified water, sodium hydroxide and non-polar macroporous adsorption resins treated with the purified water; (3) the non-polar macroporous adsorption resins are eluted with water until the solution which flows out is colorless or light brown, and is then eluted with the ethanol until no flavone flows out, and the eluent is collected; (4) the ethanol in all the eluent is evaporated and recovered, so a flavone solution is obtained; and (5) the flavone solution is concentrated and vacuum dried, so the fenugreek flavonoid extract is obtained. The method has the characteristics of quickness, high efficiency, easiness in magnification and green safety, the obtained extract has obvious blood sugar level reduction biological activity, and can be widely applied to various fenugreek flavonoids blood sugar level reduction medicines and health food development requirements.

Description

technical field [0001] The invention relates to a preparation method of a flavonoid extract, in particular to a preparation method of a fenugreek flavonoid extract and its application for lowering blood sugar. Background technique [0002] Fenugreek (Trigonella foenum-graecum L.) is an annual herbaceous plant belonging to the genus Fenugreek of the Fabaceae Papilionaceae. Its dried and mature seeds are included in the Pharmacopoeia of the People's Republic of China as a commonly used traditional Chinese medicine. Studies have shown that fenugreek contains a variety of biologically active substances, such as flavonoids, saponins, alkaloids, coumarins and polysaccharides and other active ingredients. Among them, flavonoids, as a large class of active substances, have hypoglycemic and antioxidative effects. [0003] The extraction methods of flavonoids generally adopt methods such as boiled extraction, organic solvent extraction and ultrasonic extraction. Boiled and supercrit...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61P3/10A23L1/29A61K131/00A23L33/00
Inventor 王洪伦杨仁明索有瑞
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST