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Chicken-origin H9N2 avian influenza virus strain and application thereof

An avian influenza virus, influenza virus technology, applied in the field of animal virology, can solve the problems of disease, H9N2 virus infection, threat to animal and human health, etc., to achieve the effect of increasing prevention ability and improving economic benefits

Active Publication Date: 2012-05-09
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From 2002 to 2004, there were continuous reports of H9N2 virus infecting pigs in my country and causing disease, which brought certain economic losses to the pig industry.
Then if the H9N2 subtype AIV re-excretes the new virus in pigs, it may seriously threaten the health of animals and humans

Method used

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  • Chicken-origin H9N2 avian influenza virus strain and application thereof
  • Chicken-origin H9N2 avian influenza virus strain and application thereof
  • Chicken-origin H9N2 avian influenza virus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1, Discovery and Identification of AIV-SD Strain of Avian H9N2 Subtype Influenza Virus

[0020] 1. Discovery of H9N2 subtype avian influenza virus AIV-SD strain

[0021] The inventor of the present invention isolated and obtained a strain of poultry H9N2 subtype influenza virus (Avian H9N2 Influenza A) from a certain chicken farm (in May 2010, in a chicken farm in Weifang City, Shandong Province, discovered by Liu Wenjun and Bi Yuhai). Virus), which was named AIV-SD strain, was preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures (CGMCC for short) on March 22, 2011. The address is: Courtyard 3, No. 1 Beichen West Road, Chaoyang District, Beijing No.), the deposit number is CGMCC No.4689. Avian H9N2 subtype influenza virus is also called H9N2 subtype avian influenza virus. Avian H9N2 Influenza A Virus (Avian H9N2 Influenza A Virus) AIV-SD strain CGMCC No.4689 is referred to as AIV-SD strain.

[0022] 2. Prep...

Embodiment 2

[0024] Embodiment 2, identification of avian H9N2 subtype influenza virus AIV-SD strain

[0025] 1. Hemagglutination test

[0026] Add 25 μl of 0.9% physiological saline to each well of a 96-well microcoagulation plate. Add 25 μl of the AIV-SD strain virus solution prepared in Example 1 to the wells of the first column, mix well, suck out 25 μl of the liquid from the wells of the first column and add it to the wells of the second column, and dilute sequentially until the eleventh column Aspirate 25 μl of liquid from the eleventh column well and discard. The twelfth row of wells was used as a negative control. Add 25 μl of 1% chicken erythrocyte suspension to each well, shake and mix well. Observe the result after standing at room temperature for about 30 minutes. When the negative control well is normal, the highest dilution factor that can completely agglutinate chicken red blood cells is the agglutination value of the virus fluid.

[0027] The agglutination value of the...

Embodiment 3

[0062] Embodiment 3, AIV-SD strain virus liquid EID 50 Determination of

[0063] 1. The AIV-SD strain virus solution prepared in Example 1 was diluted 10 times with sterilized PBS buffer to obtain each dilution.

[0064] 2. The diluted solution was inoculated into SPF chicken embryos (10 days old), 0.1 ml was inoculated into each chicken embryo, and the allantoic fluid was collected 72 hours after the inoculation. Three replicate experiments were set up for each dilution, that is, 3 chicken embryos were inoculated.

[0065] 3. Determination of HA titer of allantoic fluid

[0066] Add 25 μl of 0.9% physiological saline to each well of a 96-well microcoagulation plate. Add 25 μl of allantoic fluid to the wells in the first column, mix well, suck out 25 μl of liquid from the wells in the first column, add it to the wells in the second column, and dilute sequentially until the eleventh column of wells, and from the eleventh column Aspirate 25 μl of liquid from the well and dis...

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Abstract

The present invention relates to a chicken-origin H9N2 avian influenza virus strain and an application thereof. The avian H9N2 influenza A virus AIV-SD strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 22, 2011. The preservation number of the strain is CGMCC No.4689. The AIV-SD strain of the present invention provides pathogenicity for the chicken, and provides low toxicity for mammalians. After the virus solution of the AIV-SD strain is inactivated, the resulting inactivated AIV-SD strain can be used as the vaccine. After vaccinating with thevaccine of the present invention, the prevention ability of the chickens to the avian influenza can be substantially increased so as to substantially improve the economic benefits of the cultivation industry. The chicken-origin H9N2 avian influenza virus strain of the present invention provides great values for prevention and control of the avian influenza virus.

Description

technical field [0001] The invention belongs to the field of animal virology, and in particular relates to a chicken-derived H9N2 avian influenza virus strain and application thereof. Background technique [0002] Avian influenza (Avian influenza, AI) is a kind of avian (poultry and wild fowl) infectious disease syndrome caused by type A influenza virus. Influenza A viruses are divided into different subtypes according to the difference of viral hemagglutinin and neuraminidase. Since the first strain of H9N2 subtype AIV——A / Turkey / Wisconsin / 1 / 66 (Ty / WI / 1 / 66) was isolated in 1966, it has rapidly spread worldwide, mainly causing poultry respiratory tract, egg production Symptoms such as decline, individual mixed infection cases can also cause higher mortality, causing huge economic losses to the poultry industry in the world. At present, the H9N2 subtype AIV has been widely distributed in various poultry species in all continents, especially in Asia, where the H9N2 subtype AI...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N7/02A61K39/145A61P31/16C12R1/93
Inventor 刘文军范国兵毕玉海李晶张万林杨利敏孙蕾
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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