Ex-vivo nerve information dual-mode detection microelectrode array chip and preparation method thereof

Abstract

The invention discloses an ex-vivo nerve information dual mode detection microelectrode array chip and a preparation method of the ex-vivo nerve information dual mode detection microelectrode array chip, relating to the sensor technology. The chip consists of an insulation substrate, a microelectrode array, a counter electrode, a reference electrode, an electrode lead wire and a contact point and a surface insulation layer and is processed and prepared by adopting the MEMS (micro electro mechanical system) technology, wherein a microelectrode is a sensitive unit which is directly contacted with ex-vivo nerve cells of an animal, can be used for synchronous detection of transmission modes of a neural electrophysiological signal and a nervous transmitter electrochemical signal by combining the counter electrode and the reference electrode and also has the function of applying electrophotoluminescence to cells. The ex-vivo nerve information dual mode detection microelectrode array chip has function integration, is simple in circuit interface, is convenient to use and is suitable for performing dual-mode detection of ex-vivo nerve information of animals and related research in laboratories.

Description

technical field [0001] The invention relates to the technical field of microprocessing of biosensors, and relates to a microelectrode array chip for dual-mode detection of isolated nerve information and a preparation method thereof. Background technique [0002] Nerve cells are the basic structural and functional units that make up the nervous system of higher animals. Hundreds of millions of nerve cells form intricate networks through synaptic connections. Nerve cell pulse discharge and neurotransmitter release are two modes of nerve information transmission, and the two are interdependent and mutually modulated. Therefore, the synchronous detection of nerve electrophysiological signals and neurotransmitter electrochemical signals is carried out on group nerve cells to study the interaction mechanism of nerve cells, the encoding and decoding process of neural information, the pathogenesis of neuropsychiatric diseases, and drug responses. etc., have important scientific sig...

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Problems solved by technology

[0003] For a long time, people have used traditional patch clamps, glass microelectrodes, wire microelectrodes, etc. to detect the electrophysiological signals of nerve cells in vivo or in vitro. Usually, only a few channels of data can be obtained, and Electrode positioning is difficult and the operation is cumbersome; in recent years, with the development of micro-electromechanical system (MEMS) processing technology, some micro-electrode array chips made of various materials and processes have appeared at home and abroad, which can realize the electrophysiological function of group nerve cells. Synchronous detection of activity, such as the MEA chip developed by the German Multichannel company, the Michigan implantable microelectrode array produced by the American NeuroNexus company, etc., but these electrodes have not yet integrated the function of detecting neurotransmitters, and it is impossible to deeply study the neural electrophysiological signals and corresponding neurotransmitters. However, for the detection of neurotransmitter electrochemical signals, the method of large electrodes and in vitro microdialysis is mostly used at present, which has poor real-time detection and low sensitivity, and cannot realize synchronous detection with electrophysiological signals.

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