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Method for extracting chenodeoxycholic acid from goose and duck bile

A technology of chenodeoxycholic acid and duck bile, applied in the field of bioengineering, can solve the problems of poor decolorization effect and low precipitation efficiency, and achieve good decolorization effect, fast precipitation rate and improved efficiency

Inactive Publication Date: 2012-05-16
曾科
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above-mentioned problems of low precipitation efficiency and poor decolorization effect, the present invention provides a method for extracting chenodeoxycholic acid from poultry bile of factory waste, which can utilize waste, reduce costs, and expand medicinal resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A. Add 2 times the volume of sodium hydroxide solution with a mass volume ratio concentration of 50% to the bile taken, stir well and heat to 80°C, keep warm for 1 hour and cool to 50°C, adjust the pH with hydrochloric acid with a volume concentration of 20% The value is 1.2, and after standing for 3 hours, precipitation occurs;

[0022] B, filter to obtain black-green paste, add 1 times the mass, the mass volume ratio concentration is 60% sodium chloride solution, heat for 5h, let stand, filter to obtain precipitate, wash with water until pH is 6.8, dry at -10°C, Obtain the crude product of total bile acid;

[0023] C, add 95% methanol to the crude product and stir to dissolve, filter out the insoluble matter, the mass volume ratio of the crude product to 95% methanol is 1:2, add n-hexane to extract and degrease according to 1:1, take the methanol layer and adjust the pH value to 8.5 with ammonia water, Recover methanol, add water to the original volume, add barium ch...

Embodiment 2

[0026] A. Add 5 times the volume of sodium hydroxide solution with a mass volume ratio concentration of 90% to the bile taken, stir well and heat to 90°C, keep warm for 2 hours and cool to 70°C, adjust the pH with hydrochloric acid with a volume concentration of 40% Value is 5.5, stand for 5 hours, produce precipitation;

[0027] B, filter to obtain a black-green paste, add 2 times the mass, the mass volume ratio concentration is 80% sodium chloride solution and heat for 8h, let stand, filter to obtain a precipitate, wash with water until the pH is 7.5, and dry at 1°C to obtain Crude total bile acids;

[0028] C, add 95% methanol to the crude product and stir to dissolve, filter out the insoluble matter, the mass volume ratio of the crude product to 95% methanol is 1:8, add n-hexane to extract and degrease according to 1:1, take the methanol layer and use ammonia water to adjust the pH value to 8.5- 10. Recover methanol, add water to the original volume, add barium chloride a...

Embodiment 3

[0031] A. Add 3.5 times the volume of sodium hydroxide solution with a mass volume ratio concentration of 70% to the bile taken, stir well and heat to 85°C, keep it warm for 1.5 hours, then cool to 60°C, and adjust the pH with hydrochloric acid with a volume concentration of 30%. The value is 3.2, and after standing for 4 hours, precipitation occurs;

[0032] B. Filter to obtain a black-green paste, add 1.5 times the mass of sodium chloride solution with a mass volume ratio concentration of 70%, heat for 6.5 hours, let stand, filter to obtain a precipitate, wash with water until the pH is 7.2, and dry at -5°C , to obtain the crude product of total bile acid;

[0033] C, add 95% methanol to the crude product and stir to dissolve, filter out the insoluble matter, the mass volume ratio of the crude product to 95% methanol is 1:5, add n-hexane to extract and degrease according to 1:1, take the methanol layer and adjust the pH value to 9 with ammonia water, Recover methanol, add w...

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Abstract

The invention relates to a method for extracting chenodeoxycholic acid from goose and duck bile and belongs to the bioengineering field. According to the method, waste poultry bile in a factory undergoes steps of precipitation, filtering, purification and extraction and the like to extract chenodeoxycholic acid. The raw materials are abundant and low-priced. The extraction method provided by the invention has a similar effect with the extraction of ursodesoxycholic acid, can be used to utilize waste, reduce cost and enlarge medicinal resources.

Description

technical field [0001] The invention relates to a method for extracting chenodeoxycholic acid, more specifically, the invention relates to a method for extracting chenodeoxycholic acid from goose and duck bile, which belongs to the field of bioengineering. Background technique [0002] Bile acid is a sterol, which is the most abundant of the four main bile acids in humans. Glycocholic acid and taurocholic acid derived from it are the main bile acids in humans. Bile salts (sodium salts of bile acids) secreted by the liver into bile are powerful emulsifiers. After bile passes through the upper small intestine, bile salts emulsify fats so they can be digested and absorbed. After the fatty acids and glycerides of the emulsified fat droplets are absorbed by the lower small intestine, bile salts are also reabsorbed. They return to the liver and are reused, so bile salts are always circulating between the liver and the small intestine. Bile salts are especially important for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
Inventor 曾科
Owner 曾科
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