Method for inhibiting generation of aflatoxin by using D-glucal

A technology of aflatoxin and Aspergillus flavus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2013-12-18
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there is no report on the relationship between sugar utilization gene clusters and toxin synthesis

Method used

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  • Method for inhibiting generation of aflatoxin by using D-glucal
  • Method for inhibiting generation of aflatoxin by using D-glucal
  • Method for inhibiting generation of aflatoxin by using D-glucal

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Embodiment 1

[0023] Example 1, D-glucal inhibits aflatoxin production

[0024] Aspergillus flavus (CGMCC No. 3.2890) was purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences; the initiation culture of the strains: a small amount of the strains frozen in glycerol at -80°C was evenly spread on the PDA medium for activation, After culturing at 37°C for 4 days under aerobic conditions, green spores can be observed.

[0025] Spore counting: (nephelometric method) wash Aspergillus flavus spores repeatedly with 0.05% Tween20 water, the spores are finally in the form of dispersed particles, dilute in multiples, count with a hemocytometer respectively, and take 2.5ml and pour it into a cuvette, at OD600nm Measure the light absorption at , and establish a standard curve for the concentration of spores, and only need to measure the OD for the spores to be tested 600nm Light absorbance, spore number was determined by calculation. Dilute with Twee...

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Abstract

The invention discloses an application of D-glucal in inhibiting generation of aflatoxin. The invention provides an application of D-glucal in inhibiting generation of aflatoxin. The invention also provides an application of D-glucal in preparing an inhibitor for inhibiting generation of aflatoxin. Proven by experiments of the invention, D-glucal can inhibit the generation of aflatoxin and prevent aflatoxin from harming people.

Description

technical field [0001] The invention relates to a method for inhibiting the production of aflatoxin by using D-glucal. Background technique [0002] D-glucal Chinese name: D-glucal, Chinese synonym D-glucal; D-glucocene; CAS: 13265-84-4; Structural formula: [0003] D-glucal is an important raw material in organic synthesis and drug synthesis, and D-glucal is an inhibitor of glucose oxidase. [0004] Aflatoxins are among the most toxic and carcinogenic compounds found in nature. Aflatoxin contamination in agricultural products seriously threatens food safety and causes huge economic losses. Nearly half a century of research on Aspergillus flavus and toxins has elucidated the gene clusters related to toxin synthesis, and found that many external factors affect the synthesis of aflatoxin, but how do these external factors activate the intrinsic toxin synthesis of Aspergillus flavus? The pathway remains unclear. For example, carbon sources are known to play an important ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/67
Inventor 刘春明张晋丹晏石娟韩莉妲
Owner INST OF BOTANY CHINESE ACAD OF SCI
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