Microencapsulation method of triple probiotics

Abstract

A microencapsulation method of triple probiotics relates to a cell immobilization biological method. The method comprises the following steps: firstly, culturing triple probiotics to a logarithmic growth phase, preparing a soybean protein bacterial suspension; secondly, mixing the soybean protein bacterial suspension with gelatin to prepare a bacterial gel solution; thirdly, mixing the bacterial gel solution with sodium alginate, performing emulsification; fourthly, sprinkling a calcium chloride solution, performing a capsulation reaction; fifthly, spraying chitosan, performing further solidification; sixthly, washing with a calcium chloride solution, performing centrifugation, collecting capsules. The invention can perform simultaneous microencapsulation of three probiotics; A probiotic preparation prepared by the invention has very strong stress resistance, and can be colonized and grow in intestinal tracts more rapidly.

Description

technical field [0001] The invention relates to a cell immobilization biological method, which belongs to the field of probiotic products. Background technique [0002] Probiotics are one of the most important physiological flora in the intestinal tract of humans and animals, and are closely related to various physiological functions of the body. With the rapid development of microecology in recent years, a new class of drugs based on probiotics is developing rapidly. However, at present, most of the probiotic preparations developed at home and abroad are of a single type. Based on the research on the micro-ecological environment in the intestinal tract and the effect of probiotic flora, probiotic preparations have shown a tendency to develop into combined bacteria preparations in recent years. Although the development of probiotic products emerges in an endless stream, most of them have a problem that needs to be solved urgently, which is the decline of the number of viabl...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that probiotic products made by vacuum freeze-drying technology and existing microencapsulation technology have poor resistance to adverse environments such as gastric acid and bile, and most of them are of a single type of bacteria, and cannot provide nutrition for the bacteria. A method of microencapsulation of triple probiotics provided by adhering materials in the intestinal tract

[0006] The present invention can microencapsulate three kinds of probiotics at the same time, which solves the defect of traditional single-bacteria microencapsulation; the present invention uses soybean protein-sodium alginate-calcium chloride-chitosan system as the wall material to solve the problem. Probiotic products have poor resistance to external temperature changes, gastric acid, bile and other adverse environments; the wall material used in the present invention can also provide nutrition and attachment materials for probiotics, so that probiotics can quickly enter the intestinal tract after taking Colonization and growth solves the defect that traditional wall materials can only provide stress resistance