Method for identifying rice blast resistance of rice

Abstract

A method for identifying rice blast resistance of rice comprises the steps of scissoring three rice stems in a large bract period from a rice growing field, bringing the rice stems back to a laboratory, scissoring 2cm from the upper portion of each stem section and 5-6cm from the lower portion of each stem section to obtain one rice stem with the whole length of approximate 7-8cm, putting the three rice stems into culture dishes with two layers of filter paper, adding sterile water of 1mL into each culture dish, washing cultivated rice blast fungus spores with the sterile water, obtaining concentration of 106 pcs / ml, dropping spore suspending liquid of 8 muL to stem section sites of each rice stem through a liquid moving gun, putting the culture dishes into a illuminating incubator at the temperature of 26 DEG C, wrapping the culture dishes with transparent films to keep humidity in the culture dishes, cultivating for ten days in light and dark alternating modes to survey disease conditions, dividing survey disease levels into three levels, regarding the first level as disease-resistant cultivars, regarding the second level as susceptible cultivars, and regarding the third level as highly susceptible cultivars. The method optimizes the concentration and inoculation amount of inoculated rice blast fungus spores and establishes simple, convenient and accurate grading standards.

Description

technical field [0001] The patent of the present invention relates to a method for identifying the resistance of isolated rice stalks to rice blast, which is mainly used in the screening of disease resistance in the process of breeding rice varieties and new varieties. Background technique [0002] Rice blast (Magnaporthe oryzae) is the most potentially serious disease in rice production, and it has occurred in 85 rice-growing countries and regions around the world. The annual loss of rice due to rice blast accounts for about 10% to 15% of the total output, and the loss amounts to billions of dollars. Both the north and south rice regions of my country have occurred, and in the disease-endemic areas, the heavy ones can reach 40%-50%, and the particularly heavy fields even fail to harvest. [0003] In terms of disease prevention and control, comprehensive control measures based on the rational use of resistant varieties and chemical control as key technologies have been prop...

AI Technical Summary

Problems solved by technology

[0004] The existing rice blast resistance identification method, especially the identification of ear blast resistance, compared with the resistance identification method of leaf blast, the identification of ear blast The difficulty is due to the obvious difference in the heading period of different varieties and the long growth period from sowing to heading, etc., so far, there is no unified identification method for artificial inoculation at home and abroad.

Due to some defects of each, they have not been widely used. At present, the most used method is in vivo injection inoculation at the booting stage. The concentration of the bacterial solution is about 1×105 spores / mL, and the injection volume is 1.0 mL. Inoculate 20 to 30 ear buds, and investigate the incidence of rice at the yellow maturity stage

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