Traditional Chinese medicine composition for treating chicken infectious bursal disease as well as preparation method and application thereof
A technology of bursal disease and chicken infectivity, which is applied in the field of traditional Chinese medicine, can solve the problems of high yolk free cost, unstable effect, drug residue, etc., and achieve the effect of no toxic side effects, accurate and rapid curative effect, and stable quality
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[0082] The present invention also provides the preparation method of the above-mentioned Chinese medicine composition for treating chicken infectious bursal disease, which comprises:
[0083] The first step is to mix and pulverize Ligustrum lucidum and Eclipta chinensis, add ethanol to reflux and extract for 2 to 4 times, combine the reflux liquid, centrifuge, and filter to obtain the first filtrate;
[0084] In the second step, mix and pulverize dandelion, viola chinensis, bark and pulsatilla, soak in water for 20 to 30 hours, then distill, collect the distillate, and redistill the distillate to collect the first distilled liquid. The volume of the first double-distilled liquid accounts for 8 / 10-9 / 10 of the volume of the distilled liquid before double-distilled;
[0085] The third step is to mix and pulverize Nansha ginseng, Ophiopogon japonicus and Tortoise shell, add ethanol to reflux and extract for 2 to 4 times, combine the reflux liquid, centrifuge, and filter to obtain ...
Embodiment 1
[0117] Embodiment 1 acute toxicity test and long-term toxicity test
[0118]Mix and crush 30g of Ligustrum lucidum and 40g of Eclipta chinensis, add 210g of 95% ethanol to reflux and extract twice, each time for 1 hour, combine the reflux liquid, centrifuge, and filter to obtain the first filtrate; D, 10g Qinpi and 10g Pulsatilla pulsatillae were mixed and crushed, put into 160g water and soaked for 25 hours, then distilled, collected the distilled liquid, and then redistilled the distilled liquid, collected the first heavy distilled liquid, the volume of the first heavy distilled liquid was the volume of the distilled liquid 9 / 10; mix and crush 20g Nansha ginseng, 20g Ophiopogon japonicus and 5g turtle shell, add 140g 70% ethanol to reflux extraction twice, each time for 1 hour, combine the reflux liquid, centrifuge, and filter to obtain the second filtrate; 5g Astragalus and 5g of licorice were mixed and crushed, and 30g of water was added to decoct twice, and the decoction ...
Embodiment 2
[0123] The preparation of embodiment 2 Chinese medicine composition 1
[0124] Mix 300g of Ligustrum lucidum and 400g of Eclipta chinensis, add 2100g of 95% ethanol to reflux and extract twice, each time for 1 hour, combine the reflux liquid, centrifuge, and filter to obtain the first filtrate; 100g of Dandelion, 100g of Zihua D, 100g Qinpi and 100g Pulsatilla pulsatillae were mixed and crushed, put into 1600g water and soaked for 25 hours, then distilled, collected the distilled liquid, and then redistilled the distilled liquid, collected the first double distilled liquid, the volume of the first double distilled liquid was 1 / 2 of the volume of the distilled liquid 9 / 10; mix and crush 200g Nansha ginseng, 200g Ophiopogon japonicus and 50g turtle shell, add 1400g 70% ethanol to reflux extraction twice, each time for 1 hour, combine the reflux liquid, centrifuge, and filter to obtain the second filtrate; 50g Astragalus and 50g of licorice were mixed and crushed, and 300g of wat...
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