Preparation method for linear nanometre hydroxylapatite

A nano-hydroxyapatite, linear technology, applied in the field of nano-materials, can solve the problems of unsuitable for large-scale production, high price, unsuitable phage for tissue repair, etc., achieving simple preparation method, inhibition of agglomeration, mild and controllable conditions Effect

Active Publication Date: 2012-06-13
SUZHOU UNIV
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Problems solved by technology

[0005] The prior art discloses a variety of reports on the preparation of oriented hydroxyapatite by using biomimetic technology, such as the document "Bacteriophage Bundles with Prealigned Ca 2+ Initiate the Oriented Nucleation and Growth of Hydroxylapatite" pointed out that using phage bundles as templates can form oriented hydroxyapatite, but phages are not suitable for direct use in tissue repair; researchers such as Anton Ficai published in "Chemical Engineering Journal" in 2010 The literature "Self-assembled collagen / hydroxyapatite composite materials" pointed out that mineralization treatment on aligned collagen can form oriented hydroxyapatite, but it uses collagen as a template, which is expensive and not suitable for large-scale production

Method used

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  • Preparation method for linear nanometre hydroxylapatite
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  • Preparation method for linear nanometre hydroxylapatite

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[0026] The invention provides a method for preparing linear nano-hydroxyapatite, comprising the following steps:

[0027] Concentrating the silk fibroin aqueous solution of the first mass concentration to the second mass concentration and then sealing and cultivating to obtain the silk fibroin nanofiber aqueous solution;

[0028] The silk fibroin nanofiber aqueous solution is mixed with a phosphate source and a calcium source, and the linear nano-hydroxyapatite is obtained after reaction.

[0029] In the present invention, firstly, the silk fibroin aqueous solution of the first mass concentration is concentrated to the second mass concentration, and then sealed and cultivated. During this process, the silk fibroin self-assembles into silk fibroin nanofibers to obtain the silk fibroin nanofiber aqueous solution; and then Using the silk fibroin nanofiber aqueous solution as a template, nano-hydroxyapatite is synthesized on the surface of silk fibroin nanofibers. The interaction ...

Embodiment 1

[0061] The silk at a mass concentration of 0.5% Na 2 CO 3 Boil in the solution for 60 minutes at 100°C to remove sericin on the outside of the silk; wash the obtained product with deionized water, repeat 3 times, and dry at 60°C to obtain silk fibroin;

[0062] Dissolve 27 grams of silk fibroin in 100 mL of lithium bromide solution, put the obtained silk fibroin solution in a dialysis bag and immerse it in deionized water for dialysis for 3 days, during which the water is changed every two hours to obtain a pure silk fibroin solution. The mass concentration is 5.9%;

[0063] Slowly dry the silk fibroin solution at room temperature under a fume hood, increase the silk fibroin concentration to 25%, and then place it at 4°C for 2 days to obtain a silk fibroin nanofiber solution;

[0064] The silk fibroin nanofiber solution is carried out atomic force microscope analysis and infrared spectrum analysis, the results can be found in figure 1 and figure 2 , figure 1 The atomic f...

Embodiment 2

[0069] The silk at a mass concentration of 0.5% Na 2 CO 3 Boil in the solution for 60 minutes at 100°C to remove sericin on the outside of the silk; wash the obtained product with deionized water, repeat 3 times, and dry at 60°C to obtain silk fibroin;

[0070] Dissolve 27 grams of silk fibroin in 100 mL of lithium bromide solution, put the obtained silk fibroin solution in a dialysis bag and immerse it in deionized water for dialysis for 3 days, during which the water is changed every two hours to obtain a pure silk fibroin solution. The mass concentration is 5.9%;

[0071] Slowly dry the silk fibroin solution at room temperature under a fume hood, increase the silk fibroin concentration to 40%, and then place it at 4°C for 18 days to obtain a silk fibroin nanofiber solution;

[0072] The atomic force microscope analysis and infrared spectrum analysis were carried out on the silk fibroin nanofiber solution, and the results showed that the silk fibroin nanofiber was obtained...

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Abstract

The invention provides a preparation method for linear nanometre hydroxylapatite. The preparation method comprises the following steps of: concentrating silk fibroin aqueous solution of a first mass concentration to a second mass concentration, and then performing sealed cultivation to acquire the silk fibroin nanofibre aqueous solution; mixing the silk fibroin nanofiber aqueous solution with a phosphate radical source and a calcium source for reaction to acquire the linear nanometre hydroxylapatite. The invention further provides a preparation method for the nanometre hydroxylapatite. The method comprises the following steps of: firstly, concentrating silk fibroin aqueous solution of a first mass concentration to a second mass concentration, and then performing a sealed cultivation to the mixture to acquire the silk fibroin aqueous solution, wherein in the process, the silk fibroin is self-organized to be silk fibroin nanofiber, so that silk fibroin nanofiber aqueous solution is acquired ; and using the silk fibroin nanofiber aqueous solution as a template to synthetize nanometre hydroxylapatite on the surface of the silk fibroin nanofiber. The silk fibroin interacts with the hydroxylapatite, so that the union of the hydroxylapatite cannot be restrained, but also, the linear nanometre hydroxylapatite can be acquired.

Description

technical field [0001] The invention belongs to the technical field of nanometer materials, in particular to a method for preparing linear nanometer hydroxyapatite. Background technique [0002] Silk fibroin is a structural protein that exists widely in nature. It can be extracted from silk fiber of silkworm. It not only has sufficient sources and simple extraction methods, but also has good biocompatibility, biodegradability, good mechanical properties and good The adhesion, expansion and differentiation of cells is a kind of organic matrix material with good development prospects. Silk fibroin can be prepared into various forms such as solutions, films, porous scaffolds, gels, etc., and has good application prospects in the field of biomedicine. [0003] Hydroxyapatite (Hydroxyapatite, HA, Ca 10 (PO 4 ) 6 (OH) 2 ) is a weakly alkaline calcium phosphate salt slightly soluble in water, belonging to the hexagonal crystal system, and is a class of inorganic materials with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B25/32B82Y40/00
Inventor 黄晓卫吕强林莎莎张岑岑赵荟菁
Owner SUZHOU UNIV
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