Method for separating milk-derived whey protein by peg/phosphate two-phase system

A two-phase aqueous system and whey protein technology, applied in the field of PEG/phosphate two-phase aqueous system to separate milk-derived whey protein α-La and β-Lg, to achieve mild operating conditions, low price, and easy industrial production Effect

Active Publication Date: 2015-09-09
JINAN UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existing infant formula milk powder adds desalted whey powder to increase the proportion of whey protein in the milk powder to prevent infant milk allergy, so the content of β-Lg (β-lactoglobulin, β-lactoglobulin) is relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating milk-derived whey protein by peg/phosphate two-phase system
  • Method for separating milk-derived whey protein by peg/phosphate two-phase system
  • Method for separating milk-derived whey protein by peg/phosphate two-phase system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Whey protein was separated by PEG / phosphate two-phase system. The separation steps are as follows:

[0040] (1) Preparation of standard whey protein solution: standard α-La solution (1mg / mL); standard β-Lg solution (1mg / mL).

[0041] (2) Southern buffalo milk whey protein extraction: Commercially available buffalo milk was centrifuged at 4000r / min at 4°C for 20 minutes to skim, and the upper layer of fat was discarded. The skim milk was adjusted to pH 4.6 with 5M HCl, and stood for 30min at 4000r / min at 4°C. Centrifuge for 20 minutes, discard the precipitate, filter the supernatant with a 0.22 μm filter membrane, dialyze against distilled water at 4°C overnight, freeze-dry, and store in a freezer at -20°C.

[0042] (3) Use the cloud point method to draw the phase diagram of the two-phase aqueous system. The experimental method is as follows: Take a certain mass of inorganic salt (m1) and add a certain amount of distilled water (m2), dissolve, and constant volume, and c...

Embodiment 2

[0053] Embodiment 2: analyze the phase forming ability of four kinds of PEG and phosphate

[0054] In the step (3) of Example 1, the cloud point method was used to make the two-phase phase diagram of PEG1000, 1500, 4000, 6000 and dipotassium hydrogen phosphate to understand the phase-forming ability of the four PEGs and phosphate. The longer the two-node line in the aqueous two phase diagram, the stronger the phase separation ability of the two substances. The phase diagram of PEG and phosphate is as follows figure 2 shown.

Embodiment 3

[0055] Embodiment 3: Determination of PEG mass fraction in the best PEG type and system

[0056] Prepare the PEG / phosphate two-phase system with PEG mass fractions of 10.4%, 11.4%, 12.4%, 13.4%, and 14.4%, PEG types of 1000, 1500, and 4000, phosphate concentration of 14.8%, and system pH of 7.3 For the extraction effect of α-La and β-Lg, calculate the distribution coefficient and recovery rate of α-La and β-Lg respectively. The larger the ratio of the distribution coefficient of α-La and β-Lg, the better the separation effect. Best PEG species and PEG quality scores. Among them, the effect of PEG type and mass fraction on the distribution coefficient of β-Lg, and the effect of PEG type and mass fraction on the distribution coefficient of α-La are shown in Fig. image 3 and Figure 4 shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for separating milk-derived whey proteins of alpha-La and beta-Lg in a PEG / phosphate aqueous two-phase system, and belongs to the whey protein components separation technology. The milk-derived whey proteins are extracted; alpha-La and beta-Lg are separated in the PEG / phosphate aqueous two-phase system, wherein in the aqueous two-phase system, the mass fraction of PEG is 10.4%-14.4%, the concentration of phosphate is 10.8%-18.8%, and the pH of the system is 7.3; then, a coomassie brilliant blue method is used for measurement of the protein concentration, and SDS-PAGE electrophoresis is used for identification and detection. The method disclosed by the invention provides the whey protein separation technology which is cheap, simple in operation and easy to amplify and can be applied to large-scale industrialized production; and the milk-derived whey proteins of alpha-La and beta-Lg obtained by separation are applied in production of functional food and bioactivity products.

Description

technical field [0001] The invention belongs to the separation technology of whey protein components, in particular to a method for separating milk source whey protein α-La and β-Lg by a PEG / phosphate two-phase system. Background technique [0002] Dairy products are recognized as healthy foods that have a good effect on human physical and mental development because of their rich milk protein content, high nutritional value, and easy digestion and absorption by the human body. The most valuable ingredient in milk is milk protein. Contains casein and whey protein, and a small amount of fat globulin, of which whey protein accounts for about 20% of milk protein. Whey proteins, found in whey in a dissolved form, are small, compact globular proteins with a wide range of functional properties. Whey protein has the characteristics of high protein content, low cholesterol, fat and lactose content, and is easy to digest and absorb. It has high nutritional value and contains more tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K1/14
CPCC07K14/4717C07K14/76
Inventor 徐明芳成希飞李子超李昀锴向明霞
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap