Method for preparing tobacco perfume by pile-fermenting tobacco stems by utilizing resting cells

Abstract

The invention provides a method for preparing tobacco perfume by pile-fermenting tobacco stems by utilizing resting cells. Aroma-producing strains separated from the surfaces of tobacco leaves are amplified and cultured in a luria-bertani (LB) liquid culture medium and then centrifugally collected for standby application; a phosphate buffer solution is compounded, and the concentration of the buffer solution is controlled within a range that the osmotic pressure of the buffer solution can satisfy the requirement for cell growth; all the aroma-producing strains are grafted to the phosphate buffer solution and cultured for 20 to 72 hours at a temperature of 20 to 37 DEG C, and then the resting cells are manufactured for standby application; 2 to 5 times of water is added to the tobacco stems to sterilize the tobacco stems, and the resting cells are inoculated with pathogens at a proportion of 0.5 to 10 percent of the total weight of the sterilized tobacco stems; the tobacco stems and the resting cells are evenly mixed, and pile-fermented and cultured at a temperature of 20 to 37 DEG C for 24 to 120 hours after the resting cells are inoculated with the pathogens, and then a fermentation product is obtained; and finally, water is added to the fermentation product, the fermentation product is heated, extracted and concentrated, and then the tobacco perfume is manufactured. By adopting the method, wood gas in tobacco stem extracts can be effectively reduced and sweet aroma can be supplemented, so that the smoking quality of the tobacco stem extracts can be improved, the originalaroma of tobacco can be effectively promoted, stimulation can be reduced and the richness of the tobacco stem extracts can be increased.

Description

technical field [0001] The invention relates to the technical field of preparation of flavorings for tobacco, more specifically, the invention relates to a method for preparing flavorings for tobacco by using resting cells to ferment and process tobacco stems. Background technique [0002] Developing Chinese-style cigarettes, forming products with typical characteristics, improving the quality gap between Chinese-style cigarettes and foreign cigarettes, especially the lack of cigarette smoking aroma, and improving domestic and foreign market competitiveness are one of the problems that need to be solved in the development of my country's cigarette industry. one. Adding flavors and fragrances to cigarettes is an effective way to improve the smoking quality of cigarettes. Among them, the addition of tobacco-derived spices can effectively increase the content of the unique aroma of cigarettes, and at the same time reduce the tar content to a certain extent. Moreover, the additi...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a tobacco stem extract that can effectively reduce the woody gas in the tobacco stem extract and supplement elegant aromas such as sweetness, aiming at the defects of insufficient aroma and poor smoking quality of the tobacco stem extract prepared in the prior art. To improve the smoking quality of tobacco stem extracts, effectively enhance the original aroma of tobacco, reduce irritation, and increase the richness of tobacco stem extracts, the method for preparing tobacco spices by using resting cells to stack fermented tobacco stems