Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing high-intensity three-dimensional chitosan porous bracket

A technology of porous scaffold and chitosan, which is applied in the fields of medical science and prosthesis, can solve the problems of poor connectivity and poor mechanical properties of three-dimensional chitosan porous scaffold, and achieve the effects of low cost, easy promotion and simple operation

Inactive Publication Date: 2012-06-20
UNIV OF SHANGHAI FOR SCI & TECH
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current preparation method of the three-dimensional chitosan porous scaffold adopts the method of freeze-drying. In the pore-making process, only water is used as the porogen to carry out one-time freeze-drying to form the pores. Such scaffolds generally have poor connectivity between pores. , poor mechanical properties of the overall three-dimensional chitosan porous scaffold, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing high-intensity three-dimensional chitosan porous bracket
  • Method for preparing high-intensity three-dimensional chitosan porous bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A preparation method of a high-strength three-dimensional chitosan porous support, comprising the steps of:

[0034] (1) Preparation of chitosan and n-amyl alcohol emulsion

[0035] ①. Prepare 3% (W / V) chitosan acetate solution

[0036] First, measure 1ml of acetic acid and add it to a 100ml volumetric flask to make a constant volume to obtain a 1% (V / V) acetic acid solution, then weigh 3g of chitosan, and add it to a 1% (V / V) acetic acid solution to obtain 3% ( W / V) chitosan acetate solution;

[0037] The degree of deacetylation of the chitosan used is ≥91%;

[0038] ②, 1% (v / v) glutaraldehyde aqueous solution

[0039] Measure 1ml of pure glutaraldehyde and add distilled water to make up to 100ml to get 1% (v / v) glutaraldehyde aqueous solution, store at 4°C for later use;

[0040] ③ Chitosan and n-pentanol emulsion

[0041] Take 10ml of the 3% (W / V) chitosan acetic acid solution obtained in the above step ①, and carry out magnetic stirring, gradually add 20ml of n...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
porosityaaaaaaaaaa
porosityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing a high-intensity three-dimensional chitosan porous bracket. The method comprises the following steps: taking a chitosan as a raw material; taking n-amyl alcohol and water as pore-forming agents; preparing the three-dimensional chitosan porous bracket according to an emulsion freeze drying method; and utilizing a microwave oven to perform the microwave radiation treatment on the three-dimensional chitosan porous bracket, thereby finally obtaining the high-intensity three-dimensional chitosan porous bracket. The high-intensity three-dimensional chitosan porous bracket prepared according to the method for preparing the high-intensity three-dimensional chitosan porous bracket provided by the invention has a pore diameter of 20-50m and a porosity of 92%; the microwave radiation is utilized to generate a heat effect; the three-dimensional chitosan porous bracket is subjected to a cross-linking reaction, thereby forming a three-dimensional netted structure; the mechanical property of the finally obtained high-intensity three-dimensional chitosan porous bracket is obviously increased; and the prepared high-intensity three-dimensional chitosanporous bracket can be applied to the suspension culture of adherence cells.

Description

technical field [0001] The invention belongs to the field of porous material preparation, in particular to a method for preparing a high-strength three-dimensional chitosan porous support, and in particular to the field of preparing a three-dimensional porous support by an emulsion freeze-drying method. Background technique [0002] There are many methods for preparing porous scaffolds for tissue engineering, and porous leaching, freeze-drying, and emulsion freeze-drying are commonly used. Ikada et al. used water in gelatin hydrogel as a porogen, and prepared a porous scaffold by freeze-drying. However, due to the different thermal conduction rates of the hydrogel during the freezing process, the freezing rate and freezing temperature have little effect on the scaffold. Large, which makes it difficult to control the pore size of the scaffold during the preparation process, and the internal pore structure of the scaffold varies greatly. However, the scaffold prepared by the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/36C08J9/28C08L5/08A61L27/56A61L27/20
Inventor 李琳刘宝林韩宝三宋晓燕
Owner UNIV OF SHANGHAI FOR SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com