Detecting method for respiratory syncytial virus

A syncytial virus and detection method technology, which is applied in the detection field of respiratory syncytial virus, can solve the problems of high cost, low sensitivity, and low accuracy, and achieve the effects of reducing detection costs, improving sensitivity, and improving accuracy

Inactive Publication Date: 2012-06-20
ZUNYI MEDICAL UNIVERSITY
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Problems solved by technology

[0003] The technical problem to be solved by the present invention: To solve the problems of low accuracy, low sensitivity or high cost in current RSV detection, the present invention uses human endometrial adenocarcinoma cell line (JEC) as a tool to isolate RSV virus and use indirect Immunofluorescence method is a fast and sensitive detection of RSV, which improves the accuracy and sensitivity of detection and reduces the cost of detection compared with traditional methods

Method used

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Embodiment Construction

[0015] The technical proposal of the present invention is: use the endometrium adenocarcinoma cell line JEC as a respiratory syncytial virus isolation tool, and then use the indirect immunofluorescence method to detect the virus.

[0016] After recovery, JEC cells were inoculated in RPMI1640 medium containing 10% calf serum, and cultured at 95%-98% relative humidity, 37°C, 5% CO2 and 37°C. The medium was changed once every 2-3 days, and the culture was passaged once every 4-5 days. Well-grown JEC cells were digested with 0.25% trypsin to make a cell suspension, and 1×10 5 Inoculate in a 96-well culture plate at a density of 0.1 ml / well at 37°C, 5% CO 2 Culture in an incubator, and the next day, the cells adhere to the wall and cover the bottom of the well. Rinse the 96-well culture plate with PBS repeatedly three times, blot the remaining PBS solution in the well, and inoculate each well with throat swab liquid (take the throat swab in children and adults with upper respira...

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Abstract

The invention discloses a detecting method for a respiratory syncytial virus, which includes using an endometrial adenocarcinoma cell line JEC as a respiratory syncytial virus separating tool and then detecting the virus by an indirect immunofluorescence method. The detecting method can be used for visually judging whether a collected specimen contains the RSV (respiratory syncytial virus) or not, and is simple, fine in repeatability, high in sensitivity and low in cost.

Description

technical field [0001] The invention relates to a detection method for respiratory syncytial virus. Background technique [0002] Respiratory syncytial virus (RSV) belongs to the Pneumovirus genus of the Paramyxoviridae family and is one of the most common and important pathogens that cause lower respiratory tract infections in infants and young children in winter and spring. The virus is highly contagious and can easily cause disease outbreaks and severe nosocomial infections. Recently, RSV is also believed to be an important cause of severe respiratory infections in the elderly. Outbreaks in nursing homes often cause pneumonia. There is evidence that RSV infection is also associated with asthma. Falsey et al. reported that RSV infection in adults would aggravate chronic obstructive pulmonary disease; Elden et al. reported that RSV infection had a higher mortality rate in patients with immunosuppression or deficiency, bone marrow transplantation, and hematologic malignanci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N21/64
Inventor 敖弟书吴中明
Owner ZUNYI MEDICAL UNIVERSITY
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