Liquid edible fungus strain culture device and culture method

Abstract

The invention discloses a liquid edible fungus strain culture device and a liquid edible fungus strain culture method. The method comprises the following steps of: inserting a test tube for inoculation into a closed bottle plug of a primary fermentation bottle, wherein the test tube for inoculation is bottomless, the bottom of the test tube is provided with a wire net, and a breathable plug is plugged to the mouth of the tube; arranging a tee joint on a connecting tube for connecting the primary fermentation bottle and a secondary fermentation tank liquid medium inlet/outlet tube; and inoculating strains to the test tube into which the primary fermentation bottle plug is inserted, culturing sawdust strains in the test tube for inoculation, unplugging the cotton plug at the mouth of the test tube for inoculation after a large amount of young mycelia are grown, poking the sawdust strains into the primary fermentation bottle, closing the mouth of the test tube for inoculation when a large quantity of mycelium pellets appear in the bottle, pressing primary liquid seeds into a secondary fermentation tank, culturing for two to three days, and thus culturing the strains in the secondary fermentation tank successfully. A large quantity of primary liquid strains are cultured, the culture effect is extremely good, the success rate in the device is further higher than that in similar equipment at home and abroad, and the device has low price.

Description

technical field [0001] The invention relates to an optimized edible fungus liquid strain culture device and a culture method. Background technique [0002] At present, the production of edible fungi presents the development trend of industrialization, intensification and liquidization. The traditional solid culture method of edible mushrooms is not suitable for large-scale production due to the long production cycle and high labor intensity of mushroom farmers. The edible fungus liquid strain cultivation method is very suitable for large-scale industrial cultivation of edible fungi due to its short production cycle and low labor intensity for mushroom farmers. However, the current status of the use of edible mushroom liquid strains in my country is not ideal: first, the production process of liquid strains is unreasonable, and the success rate of continuous production is low; second, the price of liquid seed production equipment is too expensive, and most mushroom farmers...

AI Technical Summary

Problems solved by technology

[0003] The currently used culture method, the first-level liquid-specific cultivation method, now has three methods, one is mechanical or electromagnetic shake flask culture, which has the problems of large inoculation port, less culture amount, and poor success rate; It is to cultivate a large number of wood chip strains, directly add water, crush them and pour them into the fermentation tank for cultivation. There are problems such as large amount of species used, difficulty in testing hidden miscellaneous bacteria, and poor success rate; the third is to insert the mother species into the first-level fermentation bottle, There is a problem that the inoculation port of the fermentation bottle is large and the risk of contamination is high, and the inoculated bacteria are submerged in the liquid, and the young mycelium produced by static culture is less, which affects the quality of liquid bacteria culture

Worst of all, it is difficult to control the occurrence of contamination when inserting the tube into the fermentation bottle and fixing the stopper in the inoculation box or aseptic operation bench

There are two ways to transfer the first-level liquid seeds or special mother seeds into the second-level fermentation tank. One is to pour the first-level species into the fermentation tank with flame protection, which is difficult to operate and has a low success rate; After sterilizing and cooling, connect the primary bottle to the secondary fermenter in the inoculation box or aseptic operating table, and press the primary species into the secondary fermenter along the closed pipeline. operation, pollution is inevitable

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